Next Article in Journal
The Role of Runx2 in Microtubule Acetylation in Bone Metastatic Breast Cancer Cells
Next Article in Special Issue
Role of Sirtuins in the Pathobiology of Onco-Hematological Diseases: A PROSPERO-Registered Study and In Silico Analysis
Previous Article in Journal
The Genomic Landscape in Philadelphia-Negative Myeloproliferative Neoplasm Patients with Second Cancers
Previous Article in Special Issue
Novel Approaches in Molecular Characterization of Classical Hodgkin Lymphoma

Classifying Germinal Center Derived Lymphomas—Navigate a Complex Transcriptional Landscape

Interdisciplinary Centre for Bioinformatics, University Leipzig (IZBI), 04107 Leipzig, Germany
Fraunhofer Institute for Cell Therapy and Immunology (IZI), 04103 Leipzig, Germany
Department of Clinical Pathology, Robert-Bosch-Krankenhaus, Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, 70376 Stuttgart, Germany
Institute of Human Genetics, Ulm University and Ulm University Medical Center, 89073 Ulm, Germany
Author to whom correspondence should be addressed.
Academic Editor: Blanca Scheijen
Cancers 2022, 14(14), 3434;
Received: 3 June 2022 / Revised: 7 July 2022 / Accepted: 11 July 2022 / Published: 14 July 2022
(This article belongs to the Special Issue Molecular Characterization of Hematological Tumors)
Germinal center-derived B-cell lymphomas constitute a very heterogeneous group of neoplasms with diverse clinical presentations, prognoses, and responses to therapy. They divide into a series of subtypes, such as Diffuse Large B-cell lymphomas (DLBCL), Burkitt Lymphomas (BL), Follicular lymphomas (FL), and further, into several subclasses and rarer subtypes of finer granularity, which makes them one of the most heterogeneous cancer entities. Molecular classification schemes, first of all, derived from whole transcriptome gene expression data largely improved subtyping and functional understanding. Based on a whole transcriptome landscape of B-cell lymphoma, we show that one major caveat in the task of classification is represented by the rather fuzzy distribution of individual tumors without clear-cut borderlines between most of the subtypes, preventing their unambiguous association with clear-cut entities. This landscape is governed by the germinal center (GC) reaction and relates different subtypes to different states along the reaction path. We discuss the relatedness between the expression landscape and classifier signatures and their functional cell of origin background. This view helps to stratify lymphomas in terms of modular building blocks of signature genes and to interpret rarer subclasses in the context of larger ones.
Classification of lymphoid neoplasms is based mainly on histologic, immunologic, and (rarer) genetic features. It has been supplemented by gene expression profiling (GEP) in the last decade. Despite the considerable success, particularly in associating lymphoma subtypes with specific transcriptional programs and classifier signatures of up- or downregulated genes, competing molecular classifiers were often proposed in the literature by different groups for the same classification tasks to distinguish, e.g., BL versus DLBCL or different DLBCL subtypes. Moreover, rarer sub-entities such as MYC and BCL2 “double hit lymphomas” (DHL), IRF4-rearranged large cell lymphoma (IRF4-LCL), and Burkitt-like lymphomas with 11q aberration pattern (mnBLL-11q) attracted interest while their relatedness regarding the major classes is still unclear in many respects. We explored the transcriptional landscape of 873 lymphomas referring to a wide spectrum of subtypes by applying self-organizing maps (SOM) machine learning. The landscape reveals a continuum of transcriptional states activated in the different subtypes without clear-cut borderlines between them and preventing their unambiguous classification. These states show striking parallels with single cell gene expression of the active germinal center (GC), which is characterized by the cyclic progression of B-cells. The expression patterns along the GC trajectory are discriminative for distinguishing different lymphoma subtypes. We show that the rare subtypes take intermediate positions between BL, DLBCL, and FL as considered by the 5th edition of the WHO classification of haemato-lymphoid tumors in 2022. Classifier gene signatures extracted from these states as modules of coregulated genes are competitive with literature classifiers. They provide functional-defined classifiers with the option of consenting redundant classifiers from the literature. We discuss alternative classification schemes of different granularity and functional impact as possible avenues toward personalization and improved diagnostics of GC-derived lymphomas. View Full-Text
Keywords: molecular classifiers; gene expression; dark and light zone functions; tumor heterogeneity; subtyping; marker sets molecular classifiers; gene expression; dark and light zone functions; tumor heterogeneity; subtyping; marker sets
Show Figures

Graphical abstract

MDPI and ACS Style

Loeffler-Wirth, H.; Kreuz, M.; Schmidt, M.; Ott, G.; Siebert, R.; Binder, H. Classifying Germinal Center Derived Lymphomas—Navigate a Complex Transcriptional Landscape. Cancers 2022, 14, 3434.

AMA Style

Loeffler-Wirth H, Kreuz M, Schmidt M, Ott G, Siebert R, Binder H. Classifying Germinal Center Derived Lymphomas—Navigate a Complex Transcriptional Landscape. Cancers. 2022; 14(14):3434.

Chicago/Turabian Style

Loeffler-Wirth, Henry, Markus Kreuz, Maria Schmidt, German Ott, Reiner Siebert, and Hans Binder. 2022. "Classifying Germinal Center Derived Lymphomas—Navigate a Complex Transcriptional Landscape" Cancers 14, no. 14: 3434.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop