Store-Operated Ca2+ Entry Is Up-Regulated in Tumour-Infiltrating Lymphocytes from Metastatic Colorectal Cancer Patients
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients and Donors
2.2. Isolation of TILs and Tumour Cells
2.3. Expansion of mCRC Cells
2.4. TIL Expansion and Characterisation
2.5. Total RNA Extraction and Real-Time PCR
2.6. Immunoblotting
2.7. Solutions
2.8. [Ca2+]i Measurements
2.9. Statistics
2.10. Chemicals
3. Results
3.1. Characterisation of Ex Vivo-Expanded TILs
3.2. SOCE Is Larger in Ex Vivo-Expanded TILs
3.3. InsP3Rs- and RyRs-Mediated Ca2+ Release Result in Enhanced SOCE in Ex Vivo-Expanded TILs
3.4. Molecular Characterisation of the Ca2+ Toolkit Reveals the Up-Regulation of STIM1 Protein in Ex Vivo-Expanded TILs
3.5. Enhanced SOCE in TILs and cPBTs Is Maintained by Diacylglycerol Kinase (DGK) Activity
3.6. TCR Stimulation Induced a Larger Increase in [Ca2+]i in TILs
3.7. SOCE Tunes the Amplitude of the Ca2+ Response during Target Cell Killing
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Faris, P.; Rumolo, A.; Tapella, L.; Tanzi, M.; Metallo, A.; Conca, F.; Negri, S.; Lefkimmiatis, K.; Pedrazzoli, P.; Lim, D.; et al. Store-Operated Ca2+ Entry Is Up-Regulated in Tumour-Infiltrating Lymphocytes from Metastatic Colorectal Cancer Patients. Cancers 2022, 14, 3312. https://doi.org/10.3390/cancers14143312
Faris P, Rumolo A, Tapella L, Tanzi M, Metallo A, Conca F, Negri S, Lefkimmiatis K, Pedrazzoli P, Lim D, et al. Store-Operated Ca2+ Entry Is Up-Regulated in Tumour-Infiltrating Lymphocytes from Metastatic Colorectal Cancer Patients. Cancers. 2022; 14(14):3312. https://doi.org/10.3390/cancers14143312
Chicago/Turabian StyleFaris, Pawan, Agnese Rumolo, Laura Tapella, Matteo Tanzi, Alessia Metallo, Filippo Conca, Sharon Negri, Konstantinos Lefkimmiatis, Paolo Pedrazzoli, Dmitry Lim, and et al. 2022. "Store-Operated Ca2+ Entry Is Up-Regulated in Tumour-Infiltrating Lymphocytes from Metastatic Colorectal Cancer Patients" Cancers 14, no. 14: 3312. https://doi.org/10.3390/cancers14143312