Circular and Fusion RNAs in Medulloblastoma Development
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. RNA Sample Information
2.2. RNA-Seq Library Preparations
2.3. RNA-Seq Data Analysis
2.4. Validation of Fusion Transcripts by Sanger Sequencing
3. Results
3.1. Circular RNAs
3.2. Fusion RNAs–Cerebellum
3.3. Fusion RNAs–Medulloblastoma
4. Discussion
5. Limitations
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes in Fusion | Chromosomal Position | Exon number at Fusion Junction | Relative Orientation |
---|---|---|---|
Cerebellum-linear | |||
SEPTIN7P14--PSPH | Chr4-Chr7 | 1(NR_037630.1)-4(NM_004577.4) | NA |
FBXO25--SEPTIN14 | Chr8-Chr7 | 5(NM_183420.2)-10(NM_207366.3) | NA |
NCOA2--TRAM1 | Chr8, neighboring genes | 1(NM_006540.4)-5(NM_014294.6) | —> —> |
PAUPAR--RCN1 | Chr11, neighboring genes | 1(NR_033971.1)-2(NM_002901.4) | —> —> |
RPS10--NUDT3 | Chr6, neighboring genes | 5(NM_001014.5)-3(NM_006703.4) | —> —> |
Cerebellum-circular | |||
ADAMTSL3--SH3GL3 | Chr15, neighboring genes | 3(NM_207517.3)-7(NM_003027.5) | —> —> |
Medulloblastoma-linear | |||
KANSL1--ARL17A | Chr17, neighboring genes | 3(NM_015443.4)-3(NM_001113738.2) | —> —> |
TFG--ADGRG7 | Chr3, neighboring genes | 3(NM_006070.6)-2(NM_032787.3) | —> —> |
SPSB4--PXYLP1 | Chr3, neighboring genes | 2(NM_080862.3)-2(NM_001037172.3) | —> —> |
PVT1--CASC8 | Chr8, neighboring genes | 1(NR_003367.3)-6(NR_117100.1) | <— —> |
Medulloblastoma-circular | |||
ARL17A--KANSL1 | Chr17, neighboring genes | 3(NM_001113738.2)-3(NM_015443.4) | —> —> |
KANSL1--ARL17A | Chr17, neighboring genes | 3(NM_015443.4)-3(NM_001113738.2) | —> —> |
ARL17B--KANSL1 | Chr17, neighboring genes | 3(NM_001039083.5)-3(NM_015443.4) | —> —> |
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Azatyan, A.; Zaphiropoulos, P.G. Circular and Fusion RNAs in Medulloblastoma Development. Cancers 2022, 14, 3134. https://doi.org/10.3390/cancers14133134
Azatyan A, Zaphiropoulos PG. Circular and Fusion RNAs in Medulloblastoma Development. Cancers. 2022; 14(13):3134. https://doi.org/10.3390/cancers14133134
Chicago/Turabian StyleAzatyan, Ani, and Peter G. Zaphiropoulos. 2022. "Circular and Fusion RNAs in Medulloblastoma Development" Cancers 14, no. 13: 3134. https://doi.org/10.3390/cancers14133134