Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Murine B16 and 4T1 Tumor Models
2.3. pFUS Treatment
2.4. Proteomic Analyses
2.5. Flow Cytometric Analyses
2.6. Immunohistochemistry Analysis (IHC)
2.7. RNA-Seq
2.8. Statistical Analyses
3. Results
3.1. pFUS Effect on Tumors
3.2. Proteomic Analyses in B16 and 4T1 Flank Tumors Following pFUS
3.3. Flow Cytometric Analyses of Tumors, Inguinal Lymph Nodes, and Spleens Post-pFUS
3.4. Transcriptomic Analyses of Tumors Following pFUS
3.5. Histological Analyses of Tumors Following pFUS
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cohen, G.; Chandran, P.; Lorsung, R.M.; Aydin, O.; Tomlinson, L.E.; Rosenblatt, R.B.; Burks, S.R.; Frank, J.A. Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes. Cancers 2021, 13, 1546. https://doi.org/10.3390/cancers13071546
Cohen G, Chandran P, Lorsung RM, Aydin O, Tomlinson LE, Rosenblatt RB, Burks SR, Frank JA. Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes. Cancers. 2021; 13(7):1546. https://doi.org/10.3390/cancers13071546
Chicago/Turabian StyleCohen, Gadi, Parwathy Chandran, Rebecca M. Lorsung, Omer Aydin, Lauren E. Tomlinson, Robert B. Rosenblatt, Scott R. Burks, and Joseph A. Frank. 2021. "Pulsed-Focused Ultrasound Slows B16 Melanoma and 4T1 Breast Tumor Growth through Differential Tumor Microenvironmental Changes" Cancers 13, no. 7: 1546. https://doi.org/10.3390/cancers13071546