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Article

Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA

1
Department of Accelerator and Medical Physics, Institute for Quantum Medical Science, QST, Chiba 263-8555, Japan
2
Department of Charged Particle Therapy Research, Institute for Quantum Medical Science, QST, Chiba 263-8555, Japan
3
Veksler and Baldin Laboratory of High Energy Physics, Joint Institute for Nuclear Research, 141980 Dubna, Russia
4
Institute of System Analysis and Management, Dubna State University, 141980 Dubna, Russia
5
Medical Physics Laboratory, Medical School, University of Ioannina, 45110 Ioannina, Greece
6
Centre For Medical Radiation Physics, University of Wollongong, Wollongong 2522, Australia
7
Centre d’Études Nucléaires de Bordeaux Gradignan, CNRS/IN2P3, UMR5797, Université de Bordeaux, F-33170 Gradignan, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Michael Hausmann
Cancers 2021, 13(23), 6046; https://doi.org/10.3390/cancers13236046
Received: 6 July 2021 / Revised: 30 August 2021 / Accepted: 7 September 2021 / Published: 30 November 2021
To evaluate the repair performance of HSGc-C5 carcinoma cell against radiation-induced DNA damage, a Geant4-DNA application for radiobiological research was extended by using newly measured experimental data acquired in this study. Concerning fast- and slow-DNA rejoining, the two-lesion kinetics (TLK) model parameters were adequately optimized (the repair speeds of each process were reasonably close to the DNA rejoining speed of the nonhomologous end-joining and homologous recombination pathways). The lethality probabilities of the DNA damage induced by complex double strand breaks (DSBs) and binary repair were approximately 3% and 40%, respectively. Using the optimized repair parameters, the Geant4-DNA simulation was able to predict the cell surviving fraction (SF) and the DNA repair kinetics.
Track-structure Monte Carlo simulations are useful tools to evaluate initial DNA damage induced by irradiation. In the previous study, we have developed a Gean4-DNA-based application to estimate the cell surviving fraction of V79 cells after irradiation, bridging the gap between the initial DNA damage and the DNA rejoining kinetics by means of the two-lesion kinetics (TLK) model. However, since the DNA repair performance depends on cell line, the same model parameters cannot be used for different cell lines. Thus, we extended the Geant4-DNA application with a TLK model for the evaluation of DNA damage repair performance in HSGc-C5 carcinoma cells which are typically used for evaluating proton/carbon radiation treatment effects. For this evaluation, we also performed experimental measurements for cell surviving fractions and DNA rejoining kinetics of the HSGc-C5 cells irradiated by 70 MeV protons at the cyclotron facility at the National Institutes for Quantum and Radiological Science and Technology (QST). Concerning fast- and slow-DNA rejoining, the TLK model parameters were adequately optimized with the simulated initial DNA damage. The optimized DNA rejoining speeds were reasonably agreed with the experimental DNA rejoining speeds. Using the optimized TLK model, the Geant4-DNA simulation is now able to predict cell survival and DNA-rejoining kinetics for HSGc-C5 cells. View Full-Text
Keywords: Geant4-DNA; DNA repair; cell surviving fraction Geant4-DNA; DNA repair; cell surviving fraction
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MDPI and ACS Style

Sakata, D.; Suzuki, M.; Hirayama, R.; Abe, Y.; Muramatsu, M.; Sato, S.; Belov, O.; Kyriakou, I.; Emfietzoglou, D.; Guatelli, S.; Incerti, S.; Inaniwa, T. Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA. Cancers 2021, 13, 6046. https://doi.org/10.3390/cancers13236046

AMA Style

Sakata D, Suzuki M, Hirayama R, Abe Y, Muramatsu M, Sato S, Belov O, Kyriakou I, Emfietzoglou D, Guatelli S, Incerti S, Inaniwa T. Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA. Cancers. 2021; 13(23):6046. https://doi.org/10.3390/cancers13236046

Chicago/Turabian Style

Sakata, Dousatsu, Masao Suzuki, Ryoichi Hirayama, Yasushi Abe, Masayuki Muramatsu, Shinji Sato, Oleg Belov, Ioanna Kyriakou, Dimitris Emfietzoglou, Susanna Guatelli, Sebastien Incerti, and Taku Inaniwa. 2021. "Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA" Cancers 13, no. 23: 6046. https://doi.org/10.3390/cancers13236046

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