The mir-423-5p/MMP-2 Axis Regulates the Nerve Growth Factor-Induced Promotion of Chondrosarcoma Metastasis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Cell Migration Assay
2.4. Wound Healing Assay
2.5. Western Blot Analysis
2.6. mRNA and miRNA Quantification
2.7. Luciferase Assay
2.8. Tumor Xenograft study
2.9. Immunohistochemistry (IHC) Staining
2.10. Statistics
3. Results
3.1. NGF and MMP-2 Levels Are Positively Correlated in Human Chondrosarcoma Tissue
3.2. NGF Promotes MMP-2-Dependent Migration via the FAK and c-Src Pathways in Chondrosarcoma
3.3. The miR-423-5p/MMP-2 Axis Controls the NGF-Induced Stimulation of Chondrosarcoma Cell Migration
3.4. The Overexpression of NGF Facilitates Chondrosarcoma Metastasis of the Lungs in Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tzeng, H.-E.; Lin, S.-L.; Thadevoos, L.-A.; Ko, C.-Y.; Liu, J.-F.; Huang, Y.-W.; Lin, C.-Y.; Fong, Y.-C.; Tang, C.-H. The mir-423-5p/MMP-2 Axis Regulates the Nerve Growth Factor-Induced Promotion of Chondrosarcoma Metastasis. Cancers 2021, 13, 3347. https://doi.org/10.3390/cancers13133347
Tzeng H-E, Lin S-L, Thadevoos L-A, Ko C-Y, Liu J-F, Huang Y-W, Lin C-Y, Fong Y-C, Tang C-H. The mir-423-5p/MMP-2 Axis Regulates the Nerve Growth Factor-Induced Promotion of Chondrosarcoma Metastasis. Cancers. 2021; 13(13):3347. https://doi.org/10.3390/cancers13133347
Chicago/Turabian StyleTzeng, Huey-En, Syuan-Ling Lin, Louis-Anoop Thadevoos, Chih-Yuan Ko, Ju-Fang Liu, Yu-Wen Huang, Chih-Yang Lin, Yi-Chin Fong, and Chih-Hsin Tang. 2021. "The mir-423-5p/MMP-2 Axis Regulates the Nerve Growth Factor-Induced Promotion of Chondrosarcoma Metastasis" Cancers 13, no. 13: 3347. https://doi.org/10.3390/cancers13133347