Quantification of Differential Response of Tumour and Normal Cells to Microbeam Radiation in the Absence of FLASH Effects
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Clonogenic Assay
2.3. Irradiation Procedure
2.4. Dose Mixing
2.5. Cell Survival Analysis
3. Results
4. Discussion
Limitations of This Study
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
DVH | Dose rate volume histogram |
RT | Radiation therapy |
MRT | Microbeam radiation therapy |
BB | Broad beam |
OAR | Organ at risk |
PVDR | Peak-to-valley dose ratio |
LQ model | Linear quadratic model |
Appendix A. Tabulated Cell Survival Data
Dose [Gy] | HUVEC | MRC5 | NCI-H23 | A549 |
---|---|---|---|---|
0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 |
2 | 0.25 ± 0.03 | 0.28 ± 0.04 | 0.32 ± 0.02 | 0.55 ± 0.06 |
4 | 0.048 ± 0.019 | 0.084 ± 0.008 | 0.065 ± 0.014 | 0.17 ± 0.03 |
6 | 0.007 ± 0.002 | 0.023 ± 0.01 | 0.026 ± 0.006 | 0.056 ± 0.01 |
8 | 0.001 ± 0.0001 | 0.007 ± 0.004 | 0.003 ± 0.0002 | 0.006 ± 0.002 |
10 | 0.001 ± 0.00005 | 0.001 ± 0.00002 | 0.002 ± 0.001 |
Mean Dose [Gy] | HUVEC | MRC5 | NCI-H23 | A549 |
---|---|---|---|---|
0 | 1 ± 0 | 0.5 ± 0.25 | 1 ± 0 | 1 ± 0 |
2 | 0.57 ± 0.02 | 0.3 ± 0.14 | 0.32 ± 0.02 | 0.57 ± 0.08 |
6 | 0.13 ± 0.05 | 0.09 ± 0.02 | 0.059 ± 0.004 | 0.17 ± 0.03 |
10 | 0.06 ± 0.01 | 0.035 ± 0.011 | 0.009 ± 0.0004 | 0.049 ± 0.012 |
14 | 0.03 ± 0.01 | 0.021 ± 0.006 | 0.007 ± 0.0002 | 0.006 ± 0.004 |
18 | 0.017 ± 0.004 | 0.01 ± 0.003 | 0.003 ± 0.0002 | 0.004 ± 0.002 |
22 | 0.003 ± 0.001 | 0.002 ± 0.001 | ||
26 | 0.001 ± 0.0006 | 0.001 ± 0.0001 |
Mean Dose [Gy] | HUVEC | MRC5 | NCI-H23 | A549 |
---|---|---|---|---|
0 | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 ± 0 |
2 | 0.59 ± 0.01 | 0.42 ± 0.03 | 0.41 ± 0.02 | 0.62 ± 0.02 |
4 | 0.1 ± 0.02 | 0.23 ± 0.024 | 0.073 ± 0.001 | 0.18 ± 0.02 |
10 | 0.072 ± 0.029 | 0.15 ± 0.029 | 0.015 ± 0.002 | 0.05 ± 0.009 |
18 | 0.025 ± 0.005 | 0.072 ± 0.004 | 0.006 ± 0.0004 | 0.02 ± 0.005 |
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Cell Line | ||
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MRC5 | ||
NCI-H23 | ||
A549 | ||
HUVEC |
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Steel, H.; Brüningk, S.C.; Box, C.; Oelfke, U.; Bartzsch, S.H. Quantification of Differential Response of Tumour and Normal Cells to Microbeam Radiation in the Absence of FLASH Effects. Cancers 2021, 13, 3238. https://doi.org/10.3390/cancers13133238
Steel H, Brüningk SC, Box C, Oelfke U, Bartzsch SH. Quantification of Differential Response of Tumour and Normal Cells to Microbeam Radiation in the Absence of FLASH Effects. Cancers. 2021; 13(13):3238. https://doi.org/10.3390/cancers13133238
Chicago/Turabian StyleSteel, Harriet, Sarah C. Brüningk, Carol Box, Uwe Oelfke, and Stefan H. Bartzsch. 2021. "Quantification of Differential Response of Tumour and Normal Cells to Microbeam Radiation in the Absence of FLASH Effects" Cancers 13, no. 13: 3238. https://doi.org/10.3390/cancers13133238