Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethic Statement
2.2. Human Tissue Microarray (TMA) Immunohistochemical Analysis
2.3. Evaluation of the Human TMA Sections
2.4. Cells and Cell Culture
2.5. RNA Extraction and Real-Time PCR
2.6. Multi-Omics Analysis
2.7. Immunofluorescence
2.8. Statistical Analyses
3. Results
3.1. Types of Cervical Cancer, Mutation Load, and Copy-Number Alterations
3.2. Prognostic Value of Hub Genes
3.3. Association of the ACSS2 and PDL1 Levels with Cervical Tumorigenesis and Clinicopathological Outcomes
3.4. Correlation Analysis between ACSS2 Expression and Major Types of Cancer Immune Response
3.5. Relationship between ACSS2 and Distinct Immune Markers Sets
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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ACSS2 Expression | PDL1 Expression | ||||||
---|---|---|---|---|---|---|---|
Variables | Total | Low | High | p-Value | Low | High | p-Value |
Case number, n | 240 | 97 | 143 | - | 120 | 120 | - |
Age | 55.0 ± 13.5 | 53.4 ± 13.2 | 55.7 ± 13.7 | 0.36 | 54.1 ± 13.8 | 56.3 ± 13.1 | 0.42 |
Survive, n | 240 | 143 | 97 | 0.02 | 120 | 120 | <0.001 |
Recurrence, n | 194 | 72 | 122 | 0.39 | 108 | 86 | 0.43 |
FIGO stage, n | |||||||
I | 135 | 62 | 73 | 0.04 | 94 | 41 | <0.01 |
II | 57 | 29 | 28 | - | 27 | 30 | - |
III | 26 | 10 | 16 | - | 5 | 21 | - |
IV | 22 | 10 | 12 | - | 6 | 16 | - |
Differentiation, n | |||||||
Well | 77 | 41 | 36 | 0.03 | 11 | 66 | <0.01 |
Moderate/Poor | 163 | 61 | 102 | - | 32 | 131 | - |
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Li, C.-J.; Chiu, Y.-H.; Chang, C.; Chang, Y.-C.I.; Sheu, J.J.-C.; Chiang, A.-J. Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma. Cancers 2021, 13, 3125. https://doi.org/10.3390/cancers13133125
Li C-J, Chiu Y-H, Chang C, Chang Y-CI, Sheu JJ-C, Chiang A-J. Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma. Cancers. 2021; 13(13):3125. https://doi.org/10.3390/cancers13133125
Chicago/Turabian StyleLi, Chia-Jung, Yi-Han Chiu, Chung Chang, Yuan-Chin Ivan Chang, Jim Jinn-Chyuan Sheu, and An-Jen Chiang. 2021. "Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma" Cancers 13, no. 13: 3125. https://doi.org/10.3390/cancers13133125