Next Article in Journal
Rapid Progress in Immunotherapies for Multiple Myeloma: An Updated Comprehensive Review
Next Article in Special Issue
Therapeutic Potential of Targeting the SUMO Pathway in Cancer
Previous Article in Journal
Is Breast Cancer Risk Associated with Menopausal Hormone Therapy Modified by Current or Early Adulthood BMI or Age of First Pregnancy?
Previous Article in Special Issue
The Ubiquitin Proteasome System in Genome Stability and Cancer
 
 
Article

Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma

1
Department of Biomedical Science, Graduate School of Biomedical Science and Engineering, Hanyang University, 222 Wangsimni-ro, Seongdong, Seoul 04763, Korea
2
Biomedical Research Center, Asan Institute for Life Sciences, Seoul 05505, Korea
3
Asan Medical Center, Department of Nuclear Medicine, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa, Seoul 05505, Korea
4
College of Medicine, Hanyang University, Seoul 04763, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Tarek Abbas
Cancers 2021, 13(11), 2706; https://doi.org/10.3390/cancers13112706
Received: 5 March 2021 / Revised: 24 May 2021 / Accepted: 27 May 2021 / Published: 31 May 2021
(This article belongs to the Special Issue The Role of the Ubiquitin-Proteasome-System in Human Cancer)
Among other cancers, colorectal carcinoma (CRC) is one of the foremost causes of death worldwide. The mortality rate of those having CRC has increased dramatically in the past few years. Identification of novel regulatory molecules contributing to the progression of CRC remains a focus of significant interest. The oncogenic role of USP29 has recently been explored in a few cancer types. However, evidence concerning the expression of USP29 in other cancers is currently lacking. We identified that USP29 is highly expressed in CRC and may contribute to the progression of CRC. Depletion of USP29 in HCT116 by CRISPR-Cas9 system reduced the growth of cancer cells. Furthermore, our data suggests that USP29 knockdown reduced the tumor volume of mouse xenograft models. Future investigations are required to validate the outcome of USP29-targted therapy in patients having CRC.
Colorectal carcinoma is the third foremost cause of cancer-related deaths and accounts for 5.8% of all deaths globally. The molecular mechanisms of colon cancer progression and metastasis control are not well studied. Ubiquitin-specific protease 29 (USP29), a deubiquitinating enzyme, is involved in the occurrence and development of wide variety of cancers. However, its clinical significance and biological roles in colorectal carcinoma (CRC) remain unexplored. In this research, we observed that the rate of USP29 overexpression was higher in colon cancer patient tissues relative to its corresponding normal tissues. CRISPR-Cas9-mediated depletion of USP29 triggered DNA double strand breaks and delayed cell-cycle progression in HCT116 cells. We also demonstrated that USP29 depletion hampers the colony formation and increases apoptosis of HCT116 cells. USP29 knockdown significantly decreased CRC cell proliferation in vitro. Depletion of USP29 in HCT116 cells substantially reduced the tumor volume of mouse xenografts. In conclusion, our study shows that elevated expression of USP29 promotes malignancy in CRC, suggesting that USP29 could be a promising target for colon cancer therapy. View Full-Text
Keywords: colorectal carcinoma; deubiquitinating enzymes; ubiquitin specific protease; CRISPR-Cas9; oncogenesis; DNA damage; mouse models colorectal carcinoma; deubiquitinating enzymes; ubiquitin specific protease; CRISPR-Cas9; oncogenesis; DNA damage; mouse models
Show Figures

Figure 1

MDPI and ACS Style

Chandrasekaran, A.P.; Suresh, B.; Sarodaya, N.; Ko, N.-R.; Oh, S.-J.; Kim, K.-S.; Ramakrishna, S. Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma. Cancers 2021, 13, 2706. https://doi.org/10.3390/cancers13112706

AMA Style

Chandrasekaran AP, Suresh B, Sarodaya N, Ko N-R, Oh S-J, Kim K-S, Ramakrishna S. Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma. Cancers. 2021; 13(11):2706. https://doi.org/10.3390/cancers13112706

Chicago/Turabian Style

Chandrasekaran, Arun Pandian, Bharathi Suresh, Neha Sarodaya, Na-Re Ko, Seung-Jun Oh, Kye-Seong Kim, and Suresh Ramakrishna. 2021. "Ubiquitin Specific Protease 29 Functions as an Oncogene Promoting Tumorigenesis in Colorectal Carcinoma" Cancers 13, no. 11: 2706. https://doi.org/10.3390/cancers13112706

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop