CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Retroviral Plasmids and Infection
2.3. Western Blot
2.4. Quantitative Real-Time PCR (qPCR)
2.5. Flow Cytometry
2.6. Colony Formation Assay
2.7. Mouse Experiments
2.8. Histology and Immunohistochemistry
2.9. Statistical Analysis
3. Results
3.1. CUX1 Accelerates Pancreatic Tumorigenesis in KrasG12D Mice
3.2. CUX1 Enhances Proliferation Pathways during Tumorigenesis
3.3. p110 CUX1 Is Crucial for Enhancing KRAS-Driven Tumorigenesis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Griesmann, H.; Mühl, S.; Riedel, J.; Theuerkorn, K.; Sipos, B.; Esposito, I.; Vanden Heuvel, G.B.; Michl, P. CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation. Cancers 2021, 13, 2462. https://doi.org/10.3390/cancers13102462
Griesmann H, Mühl S, Riedel J, Theuerkorn K, Sipos B, Esposito I, Vanden Heuvel GB, Michl P. CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation. Cancers. 2021; 13(10):2462. https://doi.org/10.3390/cancers13102462
Chicago/Turabian StyleGriesmann, Heidi, Sebastian Mühl, Jan Riedel, Katharina Theuerkorn, Bence Sipos, Irene Esposito, Gregory B. Vanden Heuvel, and Patrick Michl. 2021. "CUX1 Enhances Pancreatic Cancer Formation by Synergizing with KRAS and Inducing MEK/ERK-Dependent Proliferation" Cancers 13, no. 10: 2462. https://doi.org/10.3390/cancers13102462