Establishment of Prostate Tumor Growth and Metastasis Is Supported by Bone Marrow Cells and Is Mediated by PIP5K1α Lipid Kinase
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. The Functional Consequences of the Interaction between PCa Cells and a Bone-Marrow Cells
2.2. Tumor-Associated Myeloid Cells Promote Tumor Growth and Expansion in Xenograft Mouse Model
2.3. Interaction between PCa Cells and Bone Marrow Cells Correlates with Increased BM Metastasis in a Xenograft Mouse Model
2.4. PIP5K1α Mediates Interaction between PCa Cells and Tumor-Associated Myeloid Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatments
4.2. Co-Culture of PCa Cells with U-937 Cells or Bone Marrow Cells
4.3. Fluorescence-Activated Cell Sorting (FACS) and Analysis of Co-Cultured Cells
4.4. FACS-Based Cell Cycle Analysis
4.5. Plasmids, Transfection and siRNA Knockdowns
4.6. Colony-Forming Unit (CFU) Assays Using Methylcellulose-Based Medium
4.7. Prostate Cancer Stem-Like Cell-Derived Tumor-Spheroid Formation Assay
4.8. Mouse Model of PCa Xenograft Tumors and Distant Metastases
4.9. Isolation of Bone Marrow-Derived Cells from the Xenograft Tumors
4.10. Immunoblot Analysis
4.11. Immunohistochemical Analysis
4.12. Immunofluorescence Analysis
4.13. Field-Emission Scanning Electron Microscopic Imaging of PC-3 and U-937 Cells
4.14. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Karlsson, R.; Larsson, P.; Miftakhova, R.; Syed Khaja, A.S.; Sarwar, M.; Semenas, J.; Chen, S.; Hedblom, A.; Wang, T.; Ekström-Holka, K.; et al. Establishment of Prostate Tumor Growth and Metastasis Is Supported by Bone Marrow Cells and Is Mediated by PIP5K1α Lipid Kinase. Cancers 2020, 12, 2719. https://doi.org/10.3390/cancers12092719
Karlsson R, Larsson P, Miftakhova R, Syed Khaja AS, Sarwar M, Semenas J, Chen S, Hedblom A, Wang T, Ekström-Holka K, et al. Establishment of Prostate Tumor Growth and Metastasis Is Supported by Bone Marrow Cells and Is Mediated by PIP5K1α Lipid Kinase. Cancers. 2020; 12(9):2719. https://doi.org/10.3390/cancers12092719
Chicago/Turabian StyleKarlsson, Richard, Per Larsson, Regina Miftakhova, Azharuddin Sajid Syed Khaja, Martuza Sarwar, Julius Semenas, Sa Chen, Andreas Hedblom, Tianyan Wang, Kristina Ekström-Holka, and et al. 2020. "Establishment of Prostate Tumor Growth and Metastasis Is Supported by Bone Marrow Cells and Is Mediated by PIP5K1α Lipid Kinase" Cancers 12, no. 9: 2719. https://doi.org/10.3390/cancers12092719