Next Article in Journal
Radioresistance in Glioblastoma and the Development of Radiosensitizers
Previous Article in Journal
The Optimal Duration of Adjuvant Chemotherapy in Colon Cancer
Article

RNA Sequencing of Hepatobiliary Cancer Cell Lines: Data and Applications to Mutational and Transcriptomic Profiling

1
Institute of Medical Biometry and Informatics, University of Heidelberg, 69120 Heidelberg, Germany
2
Bioinformatics Core Facility, University of Gothenburg, 40530 Gothenburg, Sweden
3
Institute of Pathology, Heidelberg University Hospital, 69120 Heidelberg, Germany
4
Department of Basic and Clinical Oncology, Faculty of Medicine, Universidad ode Chile, 8380000 Santiago, Chile
5
Department of Pathology, Faculty of Medicine, Millennium Institute of Immunology and Immunotherapy, Pontificia Universidad Católica de Chile, 8330024 Santiago, Chile
6
Deep Sequencing Core Facility, CellNetworks Excellence Cluster, University of Heidelberg, 69120 Heidelberg, Germany
7
Department of Research, Cancer Registry of Norway, 0379 Oslo, Norway
8
Department of Informatics, University of Oslo, 0373 Oslo, Norway
*
Author to whom correspondence should be addressed.
These authors share first authorship.
These authors share last authorship.
Cancers 2020, 12(9), 2510; https://doi.org/10.3390/cancers12092510
Received: 4 July 2020 / Revised: 15 August 2020 / Accepted: 19 August 2020 / Published: 3 September 2020
Research on gallbladder cancer (GBC) has been largely neglected and molecular GBC data is underrepresented in public databases. Cancer cell lines constitute a valuable tool to examine the mechanisms of malignant transformation and identify potential therapeutic targets. Here we use RNA sequencing to characterize 23 commercial hepatobiliary cancer cell lines, including ten GBC cell lines, and provide detailed mutation and gene expression data to the research community. We illustrate the practical utility of the released information by (1) assessing the presence of specific mutations in the investigated cancer cell lines, (2) comparing global gene expression patterns in cell lines and primary biliary tumours and (3) examining the expression levels of specific genes. The released data and showcase applications will ease the design of in vitro cell culture assays for future studies.
Cancer cell lines allow the identification of clinically relevant alterations and the prediction of drug response. However, sequencing data for hepatobiliary cancer cell lines in general, and particularly gallbladder cancer (GBC), are sparse. Here, we apply RNA sequencing to characterize 10 GBC, eight hepatocellular carcinoma, and five cholangiocarcinoma (CCA) cell lines. RNA extraction, quality control, library preparation, sequencing, and pre-processing of sequencing data were implemented using state-of-the-art techniques. Public data from the MSK-IMPACT database and a large cohort of Japanese biliary tract cancer patients were used to illustrate the usage of the released data. The total number of exonic mutations varied from 7207 for the cell line NOZ to 9760 for HuCCT1. Researchers planning experiments that require TP53 mutations could use the cell lines NOZ, OCUG-1, SNU308, or YoMi. Mz-Cha-1 showed mutations in ATM, SNU308 presented SMAD4 mutations, and the only investigated cell line that showed ARID1A mutations was GB-d1. SNU478 was the cell line with the global gene expression pattern most similar to GBC, intrahepatic CCA, and extrahepatic CCA. EGFR, KMT2D, and KMT2C generally presented a higher expression in the investigated cell lines than in Japanese primary GBC tumors. We provide the scientific community with detailed mutation and gene expression data, together with three showcase applications, with the aim of facilitating the design of future in vitro cell culture assays for research on hepatobiliary cancer. View Full-Text
Keywords: hepatobiliary cancer; gallbladder cancer; exome mutations; transcriptomics hepatobiliary cancer; gallbladder cancer; exome mutations; transcriptomics
Show Figures

Graphical abstract

MDPI and ACS Style

Scherer, D.; Dávila López, M.; Goeppert, B.; Abrahamsson, S.; González Silos, R.; Nova, I.; Marcelain, K.; Roa, J.C.; Ibberson, D.; Umu, S.U.; Rounge, T.B.; Roessler, S.; Lorenzo Bermejo, J. RNA Sequencing of Hepatobiliary Cancer Cell Lines: Data and Applications to Mutational and Transcriptomic Profiling. Cancers 2020, 12, 2510. https://doi.org/10.3390/cancers12092510

AMA Style

Scherer D, Dávila López M, Goeppert B, Abrahamsson S, González Silos R, Nova I, Marcelain K, Roa JC, Ibberson D, Umu SU, Rounge TB, Roessler S, Lorenzo Bermejo J. RNA Sequencing of Hepatobiliary Cancer Cell Lines: Data and Applications to Mutational and Transcriptomic Profiling. Cancers. 2020; 12(9):2510. https://doi.org/10.3390/cancers12092510

Chicago/Turabian Style

Scherer, Dominique, Marcela Dávila López, Benjamin Goeppert, Sanna Abrahamsson, Rosa González Silos, Igor Nova, Katherine Marcelain, Juan C. Roa, David Ibberson, Sinan U. Umu, Trine B. Rounge, Stephanie Roessler, and Justo Lorenzo Bermejo. 2020. "RNA Sequencing of Hepatobiliary Cancer Cell Lines: Data and Applications to Mutational and Transcriptomic Profiling" Cancers 12, no. 9: 2510. https://doi.org/10.3390/cancers12092510

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop