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Article

Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia

1
Immunology Unit, University of Extremadura, 10003 Cáceres, Spain
2
Department of Immunolgy and Allergy, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), 14004 Cordoba, Spain
3
Department of Hematology, Hospital San Pedro de Alcantara, 10003 Caceres, Spain
4
Department of Hematology, Reina Sofia University Hospital, 14004 Córdoba, Spain
5
Histology and Pathology Unit, Faculty of Veterinary, University of Extremadura, 10003 Cáceres, Spain
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Department of Immunology and Allergology, Reina Sofia University Hospital, 14004 Córdoba, Spain
7
Department of Cell Biology, Physiology and Immunology, University of Cordoba, 14004 Córdoba, Spain
*
Authors to whom correspondence should be addressed.
Cancers 2020, 12(8), 2171; https://doi.org/10.3390/cancers12082171
Received: 11 June 2020 / Revised: 25 July 2020 / Accepted: 31 July 2020 / Published: 5 August 2020
(This article belongs to the Special Issue Targeting Innate Immunity Cells in Cancer)
Background: Acute myeloid leukemia (AML) remains a major clinical challenge due to poor overall survival, which is even more dramatic in elderly patients. TIGIT, an inhibitory receptor that interacts with CD155 and CD112 molecules, is considered as a checkpoint in T and NK cell activation. This receptor shares ligands with the co-stimulatory receptor DNAM-1 and with TACTILE. The aim of this work was to analyze the expression of DNAM-1, TIGIT and TACTILE in NK cells and T cell subsets in AML patients. Methods: We have studied 36 patients at the time of diagnosis of AML and 20 healthy volunteers. The expression of DNAM-1, TIGIT and TACTILE in NK cells and T cells, according to the expression of CD3 and CD56, was performed by flow cytometry. Results: NK cells, CD56− T cells and CD56+ T (NKT-like) cells from AML patients presented a reduced expression of DNAM-1 compared with healthy volunteers. An increased expression of TIGIT was observed in mainstream CD56− T cells. No differences were observed in the expression of TACTILE. Simplified presentation of incredibly complex evaluations (SPICE) analysis of the co-expression of DNAM-1, TIGIT and TACTILE showed an increase in NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE. Low percentages of DNAM-1−TIGIT+TACTILE+ NK cells and DNAM-1− TIGIT+TACTILE+ CD56− T cells were associated with a better survival of AML patients. Conclusions: The expression of DNAM-1 is reduced in NK cells and in CD4+ and CD8+ T cells from AML patients compared with those from healthy volunteers. An increased percentage of NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE is associated with patient survival, supporting the role of TIGIT as a novel candidate for checkpoint blockade. View Full-Text
Keywords: acute myeloid leukemia; DNAM-1; NK cells; NKT-like cells; TACTILE; T cells; TIGIT acute myeloid leukemia; DNAM-1; NK cells; NKT-like cells; TACTILE; T cells; TIGIT
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MDPI and ACS Style

Valhondo, I.; Hassouneh, F.; Lopez-Sejas, N.; Pera, A.; Sanchez-Correa, B.; Guerrero, B.; Bergua, J.M.; Arcos, M.J.; Bañas, H.; Casas-Avilés, I.; Sanchez-Garcia, J.; Serrano, J.; Martin, C.; Duran, E.; Alonso, C.; Solana, R.; Tarazona, R. Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia. Cancers 2020, 12, 2171. https://doi.org/10.3390/cancers12082171

AMA Style

Valhondo I, Hassouneh F, Lopez-Sejas N, Pera A, Sanchez-Correa B, Guerrero B, Bergua JM, Arcos MJ, Bañas H, Casas-Avilés I, Sanchez-Garcia J, Serrano J, Martin C, Duran E, Alonso C, Solana R, Tarazona R. Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia. Cancers. 2020; 12(8):2171. https://doi.org/10.3390/cancers12082171

Chicago/Turabian Style

Valhondo, Isabel, Fakhri Hassouneh, Nelson Lopez-Sejas, Alejandra Pera, Beatriz Sanchez-Correa, Beatriz Guerrero, Juan M. Bergua, Maria Jose Arcos, Helena Bañas, Ignacio Casas-Avilés, Joaquin Sanchez-Garcia, Josefina Serrano, Carmen Martin, Esther Duran, Corona Alonso, Rafael Solana, and Raquel Tarazona. 2020. "Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia" Cancers 12, no. 8: 2171. https://doi.org/10.3390/cancers12082171

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