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Article

LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma

1
Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Av. de Fuente Nueva s/n, 18071 Granada, Spain
2
GENYO, Centre for Genomics and Oncological Research, Pfizer/University of Granada/Andalusian Regional Government, Av. de la Ilustración 114, 18007 Granada, Spain
3
Department of Biochemistry and Molecular Biology III and Immunology, Faculty of Medicine, University of Granada, Av. de la Investigación 11, 18007 Granada, Spain
4
Health Research Institute of Granada (ibs.Granada), Av. Fuerzas Armadas 2, 18014 Granada, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cancers 2020, 12(8), 2080; https://doi.org/10.3390/cancers12082080
Received: 13 July 2020 / Accepted: 24 July 2020 / Published: 28 July 2020
(This article belongs to the Collection Regulatory and Non-Coding RNAs in Cancer Epigenetic Mechanisms)
Long non-coding RNAs (lncRNAs) are a heterogeneous class of non-coding RNAs whose biological roles are still poorly understood. LncRNAs serve as gene expression regulators, frequently interacting with epigenetic factors to shape the outcomes of crucial biological processes, and playing roles in different pathologies including cancer. Over the last years, growing scientific evidence supports the key role of some lncRNAs in tumor development and proposes them as valuable biomarkers for the clinic. In this study, we aimed to characterize lncRNAs whose expression is altered in tumor samples from patients with lung adenocarcinoma (LUAD) compared to adjacent normal tissue samples. On an RT-qPCR survey of 90 cancer-related lncRNAs, we found one lncRNA, DLG2-AS1, which was consistently downregulated in 70 LUAD patients. To gain insight into its biological function, DLG2-AS1 was cloned and successfully re-expressed in LUAD cancer cell lines. We determined that DLG2-AS1 is not a cis-regulatory element of its overlapping gene DLG2, as their transcription levels were not correlated, nor did DLG2-AS1 restoration modify the expression of DLG2 protein. Furthermore, after generating a receiver operating curve (ROC) and calculating the area under curve (AUC), we found that DLG2-AS1 expression showed high sensitivity and specificity (AUC = 0.726) for the classification of LUAD and normal samples, determining its value as a potential lung cancer biomarker. View Full-Text
Keywords: adenocarcinoma of lung; biomarkers; tumor; RNA; long noncoding adenocarcinoma of lung; biomarkers; tumor; RNA; long noncoding
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MDPI and ACS Style

Arenas, A.M.; Cuadros, M.; Andrades, A.; García, D.J.; Coira, I.F.; Rodríguez, M.I.; Baliñas-Gavira, C.; Peinado, P.; Álvarez-Pérez, J.C.; Medina, P.P. LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma. Cancers 2020, 12, 2080. https://doi.org/10.3390/cancers12082080

AMA Style

Arenas AM, Cuadros M, Andrades A, García DJ, Coira IF, Rodríguez MI, Baliñas-Gavira C, Peinado P, Álvarez-Pérez JC, Medina PP. LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma. Cancers. 2020; 12(8):2080. https://doi.org/10.3390/cancers12082080

Chicago/Turabian Style

Arenas, Alberto M., Marta Cuadros, Alvaro Andrades, Daniel J. García, Isabel F. Coira, María I. Rodríguez, Carlos Baliñas-Gavira, Paola Peinado, Juan C. Álvarez-Pérez, and Pedro P. Medina 2020. "LncRNA DLG2-AS1 as a Novel Biomarker in Lung Adenocarcinoma" Cancers 12, no. 8: 2080. https://doi.org/10.3390/cancers12082080

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