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Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines

1
Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, TWIns 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan
2
Department of Biomolecular Profiling, Translational Research Center, Fukushima Medical University, Hikarigaoka, Fukushima 960-1295, Japan
3
Department of Cell Factory, Translational Research Center, Fukushima Medical University, Hikarigaoka, Fukushima 960-1295, Japan
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(7), 1811; https://doi.org/10.3390/cancers12071811
Received: 9 June 2020 / Revised: 25 June 2020 / Accepted: 3 July 2020 / Published: 6 July 2020
(This article belongs to the Section Cancer Therapy)
HER2 is overexpressed in 25–30% of breast cancers, and approximately 30% of HER2-positive breast cancers metastasize to the brain. Although the incidence of brain metastasis in HER2-positive breast cancer is high, previous studies have been mainly based on cell lines of the triple-negative subtype, and the molecular mechanisms of brain metastasis in HER2-positive breast cancer are unclear. In the present study, we performed intracranial injection using nine HER2-positive breast cancer cell lines to evaluate their proliferative activity in brain tissue. Our results show that UACC-893 and MDA-MB-453 cells rapidly proliferated in the brain parenchyma, while the other seven cell lines moderately or slowly proliferated. Among these nine cell lines, the proliferative activity in brain tissue was not correlated with either the HER2 level or the HER2 phosphorylation status. To extract signature genes associated with brain colonization, we conducted microarray analysis and found that these two cell lines shared 138 gene expression patterns. Moreover, some of these genes were correlated with poor prognosis in HER2-positive breast cancer patients. Our findings might be helpful for further studying brain metastasis in HER2-positive breast cancer. View Full-Text
Keywords: HER2-positive breast cancer; brain metastasis; intracranial injection; in vivo imaging; microarray HER2-positive breast cancer; brain metastasis; intracranial injection; in vivo imaging; microarray
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MDPI and ACS Style

Kuroiwa, Y.; Nakayama, J.; Adachi, C.; Inoue, T.; Watanabe, S.; Semba, K. Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines. Cancers 2020, 12, 1811. https://doi.org/10.3390/cancers12071811

AMA Style

Kuroiwa Y, Nakayama J, Adachi C, Inoue T, Watanabe S, Semba K. Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines. Cancers. 2020; 12(7):1811. https://doi.org/10.3390/cancers12071811

Chicago/Turabian Style

Kuroiwa, Yuka, Jun Nakayama, Chihiro Adachi, Takafumi Inoue, Shinya Watanabe, and Kentaro Semba. 2020. "Proliferative Classification of Intracranially Injected HER2-positive Breast Cancer Cell Lines" Cancers 12, no. 7: 1811. https://doi.org/10.3390/cancers12071811

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