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Article

The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles

1
Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy
2
Department of Chemistry, Biology and Biotechnology, University of Perugia, Via del Giochetto, 06122 Perugia, Italy
*
Author to whom correspondence should be addressed.
Cancers 2020, 12(4), 900; https://doi.org/10.3390/cancers12040900
Received: 22 January 2020 / Revised: 7 February 2020 / Accepted: 31 March 2020 / Published: 7 April 2020
A new pathway leading to the n-10 fatty acid series has been recently evidenced, starting from sapienic acid, a monounsaturated fatty acid (MUFA) resulting from the transformation of palmitic acid by delta-6 desaturase. Sapienic acid has attracted attention as a novel marker of cancer cell plasticity. Here, we analyzed fatty acids, including the n-10 fatty acid contents, and for the first time, compared cell membranes and the corresponding extracellular vesicles (EV) of two human prostatic adenocarcinoma cell lines of different aggressiveness (PC3 and LNCaP). The n-10 components were 9–13% of the total fatty acids in both cancer cell lines and EVs, with total MUFA levels significantly higher in EVs of the most aggressive cell type (PC3). High sapienic/palmitoleic ratios indicated the preference for delta-6 versus delta-9 desaturase enzymatic activity in these cell lines. The expressions analysis of enzymes involved in desaturation and elongation by qRT-PCR showed a higher desaturase activity in PC3 and a higher elongase activity toward polyunsaturated fatty acids than toward saturated fatty acids, compared to LNCaP cells. Our results improve the present knowledge in cancer fatty acid metabolism and lipid phenotypes, highlighting EV lipidomics to monitor positional fatty acid isomer profiles and MUFA levels in cancer. View Full-Text
Keywords: sebaleic acid; sapienic acid; positional fatty acid isomer; trans geometrical isomer; extracellular vesicle lipidome; desaturase enzyme; elongase enzyme; lipidomics sebaleic acid; sapienic acid; positional fatty acid isomer; trans geometrical isomer; extracellular vesicle lipidome; desaturase enzyme; elongase enzyme; lipidomics
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MDPI and ACS Style

Ferreri, C.; Sansone, A.; Buratta, S.; Urbanelli, L.; Costanzi, E.; Emiliani, C.; Chatgilialoglu, C. The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles. Cancers 2020, 12, 900. https://doi.org/10.3390/cancers12040900

AMA Style

Ferreri C, Sansone A, Buratta S, Urbanelli L, Costanzi E, Emiliani C, Chatgilialoglu C. The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles. Cancers. 2020; 12(4):900. https://doi.org/10.3390/cancers12040900

Chicago/Turabian Style

Ferreri, Carla, Anna Sansone, Sandra Buratta, Lorena Urbanelli, Eva Costanzi, Carla Emiliani, and Chryssostomos Chatgilialoglu. 2020. "The n-10 Fatty Acids Family in the Lipidome of Human Prostatic Adenocarcinoma Cell Membranes and Extracellular Vesicles" Cancers 12, no. 4: 900. https://doi.org/10.3390/cancers12040900

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