Oxaliplatin-Induced DHX9 Phosphorylation Promotes Oncogenic Circular RNA CCDC66 Expression and Development of Chemoresistance
Abstract
:1. Introduction
2. Results
2.1. The Expression Level of circCCDC66 Is Elevated in the Oxaliplatin-Resistant Colorectal Cancer Cells
2.2. The Elevated circCCDC66 Is Required for Cell Survival Against Oxaliplatin-Induced Apoptosis
2.3. The Expression of circCCDC66 Is Induced by Treatment with Oxaliplatin
2.4. Oxaliplatin Promotes circCCDC66 Expression through DHX9 Phosphorylation
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Treatment
4.2. RNA Preparation, Reverse Transcription and Quantitative PCR
4.3. Transfection
4.4. Cell Survival and Caspase 3 Activity Assays
4.5. Clonogenic Assay
4.6. Immunoprecipitation and Immunoblot
4.7. RNA Sequencing and Bioinformatic Analyses
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lin, Y.-C.; Yu, Y.-S.; Lin, H.-H.; Hsiao, K.-Y. Oxaliplatin-Induced DHX9 Phosphorylation Promotes Oncogenic Circular RNA CCDC66 Expression and Development of Chemoresistance. Cancers 2020, 12, 697. https://doi.org/10.3390/cancers12030697
Lin Y-C, Yu Y-S, Lin H-H, Hsiao K-Y. Oxaliplatin-Induced DHX9 Phosphorylation Promotes Oncogenic Circular RNA CCDC66 Expression and Development of Chemoresistance. Cancers. 2020; 12(3):697. https://doi.org/10.3390/cancers12030697
Chicago/Turabian StyleLin, Ya-Chi, Ya-Shan Yu, Hui-Hsuan Lin, and Kuei-Yang Hsiao. 2020. "Oxaliplatin-Induced DHX9 Phosphorylation Promotes Oncogenic Circular RNA CCDC66 Expression and Development of Chemoresistance" Cancers 12, no. 3: 697. https://doi.org/10.3390/cancers12030697