The Second Generation Antibody-Drug Conjugate SYD985 Overcomes Resistances to T-DM1
Abstract
:1. Introduction
2. Results
2.1. Models of Resistance to T-DM1
2.2. Characterization of in vitro Resistant Models
2.3. Response of T-DM1-Resistant Models to SYD985
3. Discussion
4. Material and Methods
4.1. Reagents
4.2. Tumor Samples
4.3. Patient-Derived Xenografts (PDXs)
4.4. In Vivo Treatments
4.5. Primary Cultures
4.6. Generation of T-DM1 Resistant Models
4.7. Plasmids and shRNA Lentiviruses
4.8. Immunohistochemical Analysis
4.9. T-DM1 Labeling and Analysis of Lysosomal Activity
4.10. mRNA Expression
4.11. Cell Proliferation Assay
4.12. Protein Extraction and Immunoblotting
4.13. Copy Number Analysis
4.14. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nadal-Serrano, M.; Morancho, B.; Escrivá-de-Romaní, S.; Bernadó Morales, C.; Luque, A.; Escorihuela, M.; Espinosa Bravo, M.; Peg, V.; Dijcks, F.A.; Dokter, W.H.A.; et al. The Second Generation Antibody-Drug Conjugate SYD985 Overcomes Resistances to T-DM1. Cancers 2020, 12, 670. https://doi.org/10.3390/cancers12030670
Nadal-Serrano M, Morancho B, Escrivá-de-Romaní S, Bernadó Morales C, Luque A, Escorihuela M, Espinosa Bravo M, Peg V, Dijcks FA, Dokter WHA, et al. The Second Generation Antibody-Drug Conjugate SYD985 Overcomes Resistances to T-DM1. Cancers. 2020; 12(3):670. https://doi.org/10.3390/cancers12030670
Chicago/Turabian StyleNadal-Serrano, Mercedes, Beatriz Morancho, Santiago Escrivá-de-Romaní, Cristina Bernadó Morales, Antonio Luque, Marta Escorihuela, Martín Espinosa Bravo, Vicente Peg, Fred A. Dijcks, Wim H.A. Dokter, and et al. 2020. "The Second Generation Antibody-Drug Conjugate SYD985 Overcomes Resistances to T-DM1" Cancers 12, no. 3: 670. https://doi.org/10.3390/cancers12030670
APA StyleNadal-Serrano, M., Morancho, B., Escrivá-de-Romaní, S., Bernadó Morales, C., Luque, A., Escorihuela, M., Espinosa Bravo, M., Peg, V., Dijcks, F. A., Dokter, W. H. A., Cortés, J., Saura, C., & Arribas, J. (2020). The Second Generation Antibody-Drug Conjugate SYD985 Overcomes Resistances to T-DM1. Cancers, 12(3), 670. https://doi.org/10.3390/cancers12030670