Mapping the Relative Biological Effectiveness of Proton, Helium and Carbon Ions with High-Throughput Techniques
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Physical Setup and Charged Particle Irradiation Strategy
4.2. Cell Culture
4.3. High-Throughput Clonogenic Assay
4.4. Surviving Fraction Analysis and RBE Calculation
4.5. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Column | Helium Ions | Carbon Ions | ||||
---|---|---|---|---|---|---|
yd, keV/µm | RBE (0.5 SF) * | RBE (0.1 SF) * | yd, keV/µm | RBE (0.5 SF) * | RBE (0.1 SF) * | |
1 | 10.4 | 1.24 | ** | 18.6 | 1.51 | 1.60 |
2 | 13.8 | 1.34 | 1.58 | 36.2 | 2.31 | 2.29 |
3 | 19.1 | 1.37 | 1.75 | 55.6 | 4.02 | 3.55 |
4 | 27.9 | 1.36 | 1.70 | 61.3 | 3.99 | 3.55 |
5 | 42.0 | 1.54 | 1.88 | 72.6 | 4.57 | 4.15 |
6 | 51.4 | 2.03 | 2.26 | 87.9 | 5.24 | 4.28 |
7 | 62.0 | 2.74 | 2.70 | 113.8 | 4.51 | 3.96 |
8 | 70.8 | 3.12 | 3.47 | 146.0 | 4.59 | 3.65 |
9 | 79.0 | 4.06 | 4.13 | 181.3 | 3.61 | 3.05 |
10 | 84.9 | 3.89 | 4.25 | 230.8 | 3.16 | 2.49 |
11 | 88.0 | 4.10 | 3.62 | 263.6 | 2.06 | 1.60 |
12 | 84.7 | 2.97 | ** | 270.3 | 0.68 | ** |
Column | Helium Ions | Carbon Ions | ||||
---|---|---|---|---|---|---|
yd, keV/µm | RBE (0.5 SF) * | RBE (0.1 SF) * | yd, keV/µm | RBE (0.5 SF) * | RBE (0.1 SF) * | |
1 | 10.4 | ** | ** | 18.6 | 1.61 | ** |
2 | 13.8 | 0.61 | ** | 36.2 | 2.22 | 1.79 |
3 | 19.1 | 1.00 | ** | 55.6 | 3.50 | 2.46 |
4 | 27.9 | 1.61 | 0.96 | 61.3 | 3.82 | 2.26 |
5 | 42.0 | 1.93 | 1.43 | 72.6 | 4.31 | 2.53 |
6 | 51.4 | 2.60 | 1.81 | 87.9 | 4.81 | 2.82 |
7 | 62.0 | 2.89 | 2.24 | 113.8 | 4.56 | 2.67 |
8 | 70.8 | 4.41 | 2.91 | 146.0 | 4.31 | 2.52 |
9 | 79.0 | 5.44 | 3.19 | 181.3 | 3.76 | 2.21 |
10 | 84.9 | 3.97 | 2.33 | 230.8 | 3.40 | 1.99 |
11 | 88.0 | 1.76 | ** | 263.6 | 2.12 | 1.24 |
12 | 84.7 | ** | ** | 270.3 | 0.95 | ** |
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Bronk, L.; Guan, F.; Patel, D.; Ma, D.; Kroger, B.; Wang, X.; Tran, K.; Yiu, J.; Stephan, C.; Debus, J.; et al. Mapping the Relative Biological Effectiveness of Proton, Helium and Carbon Ions with High-Throughput Techniques. Cancers 2020, 12, 3658. https://doi.org/10.3390/cancers12123658
Bronk L, Guan F, Patel D, Ma D, Kroger B, Wang X, Tran K, Yiu J, Stephan C, Debus J, et al. Mapping the Relative Biological Effectiveness of Proton, Helium and Carbon Ions with High-Throughput Techniques. Cancers. 2020; 12(12):3658. https://doi.org/10.3390/cancers12123658
Chicago/Turabian StyleBronk, Lawrence, Fada Guan, Darshana Patel, Duo Ma, Benjamin Kroger, Xiaochun Wang, Kevin Tran, Joycelyn Yiu, Clifford Stephan, Jürgen Debus, and et al. 2020. "Mapping the Relative Biological Effectiveness of Proton, Helium and Carbon Ions with High-Throughput Techniques" Cancers 12, no. 12: 3658. https://doi.org/10.3390/cancers12123658