Evidences of a Direct Relationship between Cellular Fuel Supply and Ciliogenesis Regulated by Hypoxic VDAC1-ΔC
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.1.1. Pharmacological Inhibitors and Chemicals
2.1.2. RNA Interference
2.1.3. Mutations and Stable Transfections
2.1.4. Colony-Forming Assay
2.1.5. Respirometry and Extracellular Acidification
2.1.6. Phenotype MicroArray on Omnilog™ Analyser
2.1.7. ATP Determination
2.1.8. Lactate Measurement
2.1.9. FACS Analysis
2.1.10. Microtubule Assay
2.1.11. Electron Microscopy
2.2. Immunoblotting
Co-Immunoprecipitation Assay
2.3. Immunocytochemistry
2.3.1. Microarray Experiments
2.3.2. Statistics
3. Results
3.1. Genetic Proof of Concept in Cellular Model
3.2. Metabolic Adaptability is Controlled by VDAC1-ΔC
3.3. Mitochondrial VDAC1-ΔC Represses Ciliogenesis
3.4. When VDAC1 Meets Tubulin to Control Primary Cilium and Metabolism
3.5. Direct Impact of VDAC1-ΔC on Cell Survival
3.6. Direct Impact of VDAC1-ΔC on Cell Metabolism
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Meyenberg Cunha-de Padua, M.; Fabbri, L.; Dufies, M.; Lacas-Gervais, S.; Contenti, J.; Voyton, C.; Fazio, S.; Irondelle, M.; Mograbi, B.; Rouleau, M.; et al. Evidences of a Direct Relationship between Cellular Fuel Supply and Ciliogenesis Regulated by Hypoxic VDAC1-ΔC. Cancers 2020, 12, 3484. https://doi.org/10.3390/cancers12113484
Meyenberg Cunha-de Padua M, Fabbri L, Dufies M, Lacas-Gervais S, Contenti J, Voyton C, Fazio S, Irondelle M, Mograbi B, Rouleau M, et al. Evidences of a Direct Relationship between Cellular Fuel Supply and Ciliogenesis Regulated by Hypoxic VDAC1-ΔC. Cancers. 2020; 12(11):3484. https://doi.org/10.3390/cancers12113484
Chicago/Turabian StyleMeyenberg Cunha-de Padua, Monique, Lucilla Fabbri, Maeva Dufies, Sandra Lacas-Gervais, Julie Contenti, Charles Voyton, Sofia Fazio, Marie Irondelle, Baharia Mograbi, Matthieu Rouleau, and et al. 2020. "Evidences of a Direct Relationship between Cellular Fuel Supply and Ciliogenesis Regulated by Hypoxic VDAC1-ΔC" Cancers 12, no. 11: 3484. https://doi.org/10.3390/cancers12113484