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Open AccessArticle

Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics

1
ARC Centre of Excellence for Nanoscale BioPhotonics, MQ Photonics, Macquarie University, 2109 Sydney, Australia
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Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 119991 Moscow, Russia
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Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, 2109 Sydney, Australia
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School of Biomedical Engineering, University of Technology Sydney, 2007 Sydney, Australia
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Department of Molecular Sciences, Macquarie University, 2109 Sydney, Australia
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Minomic International Ltd., 2113 Sydney, Australia
*
Authors to whom correspondence should be addressed.
Cancers 2020, 12(1), 81; https://doi.org/10.3390/cancers12010081
Received: 2 December 2019 / Revised: 22 December 2019 / Accepted: 24 December 2019 / Published: 29 December 2019
During the last decade, isolation of circulating tumour cells via blood liquid biopsy of prostate cancer (PCa) has attracted significant attention as an alternative, or substitute, to conventional diagnostic tests. However, it was previously determined that localised forms of PCa shed a small number of cancer cells into the bloodstream, and a large volume of blood is required just for a single test, which is impractical. To address this issue, urine has been used as an alternative to blood for liquid biopsy as a truly non-invasive, patient-friendly test. To this end, we developed a spiral microfluidic chip capable of isolating PCa cells from the urine of PCa patients. Potential clinical utility of the chip was demonstrated using anti-Glypican-1 (GPC-1) antibody as a model of the primary antibody in immunofluorescent assay for identification and detection of the collected tumour cells. The microchannel device was first evaluated using DU-145 cells in a diluted Dulbecco’s phosphate-buffered saline sample, where it demonstrated >85 (±6) % efficiency. The microchannel proved to be functional in at least 79% of cases for capturing GPC1+ putative tumour cells from the urine of patients with localised PCa. More importantly, a correlation was found between the amount of the captured GPC1+ cells and crucial diagnostic and prognostic parameter of localised PCa—Gleason score. Thus, the technique demonstrated promise for further assessment of its diagnostic value in PCa detection, diagnosis, and prognosis. View Full-Text
Keywords: prostate cancer; inertial microfluidics; cell separation; tumour cells; glycoprotein prostate cancer; inertial microfluidics; cell separation; tumour cells; glycoprotein
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MDPI and ACS Style

Rzhevskiy, A.S.; Razavi Bazaz, S.; Ding, L.; Kapitannikova, A.; Sayyadi, N.; Campbell, D.; Walsh, B.; Gillatt, D.; Ebrahimi Warkiani, M.; Zvyagin, A.V. Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics. Cancers 2020, 12, 81. https://doi.org/10.3390/cancers12010081

AMA Style

Rzhevskiy AS, Razavi Bazaz S, Ding L, Kapitannikova A, Sayyadi N, Campbell D, Walsh B, Gillatt D, Ebrahimi Warkiani M, Zvyagin AV. Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics. Cancers. 2020; 12(1):81. https://doi.org/10.3390/cancers12010081

Chicago/Turabian Style

Rzhevskiy, Alexey S.; Razavi Bazaz, Sajad; Ding, Lin; Kapitannikova, Alina; Sayyadi, Nima; Campbell, Douglas; Walsh, Bradley; Gillatt, David; Ebrahimi Warkiani, Majid; Zvyagin, Andrei V. 2020. "Rapid and Label-Free Isolation of Tumour Cells from the Urine of Patients with Localised Prostate Cancer Using Inertial Microfluidics" Cancers 12, no. 1: 81. https://doi.org/10.3390/cancers12010081

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