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Article

Soluble HLA in the Aqueous Humour of Uveal Melanoma Is Associated with Unfavourable Tumour Characteristics

1
Department of Ophthalmology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
2
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, 2333ZA Leiden, The Netherlands
3
Department of Pathology, Leiden University Medical Center, 2333ZA Leiden, The Netherlands
4
Department of Pathology, Section Ophthalmic Pathology, Erasmus MC University Medical Center, 3015GD Rotterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(8), 1202; https://doi.org/10.3390/cancers11081202
Received: 25 June 2019 / Revised: 9 August 2019 / Accepted: 13 August 2019 / Published: 18 August 2019
(This article belongs to the Special Issue Uveal Melanoma)
A high HLA expression in uveal melanoma (UM) is part of the prognostically unfavorable inflammatory phenotype. We wondered whether the presence of soluble HLA (sHLA) in the aqueous humour is associated with clinical, histopathological or genetic tumour characteristics, and represents tumour HLA expression and intratumoural inflammation. Aqueous humour from 108 UM patients was analysed for the presence of sHLA, using a Luminex assay specific for HLA Class I. Clinical and genetic parameters were compared between sHLA-positive and negative eyes. A qPCR analysis was performed on tumour tissue using a Fluidigm assay. In 19/108 UM-containing eyes, the sHLA level in the aqueous was above the detection limit. Tumours in sHLA-positive eyes were significantly larger, more frequently involved the ciliary body, and more often showed monosomy 3, gain of chromosome 8q and loss of BAP1 staining. Melanoma-related survival was worse in patients with sHLA-positive aqueous humour. sHLA in the aqueous did not represent the tumour’s HLA expression and did not relate to immune cell infiltration in the tumour. We conclude that UM-containing eyes may contain sHLA in the aqueous humour, where it is a prognostically-unfavourable sign and may influence local immune responses. View Full-Text
Keywords: uveal melanoma; soluble HLA; infiltrate; aqueous humour; liquid biopsies; immunotherapy uveal melanoma; soluble HLA; infiltrate; aqueous humour; liquid biopsies; immunotherapy
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MDPI and ACS Style

Wierenga, A.P.A.; Gezgin, G.; van Beelen, E.; Eikmans, M.; Spruyt-Gerritse, M.; Brouwer, N.J.; Versluis, M.; Verdijk, R.M.; van Duinen, S.G.; Marinkovic, M.; Luyten, G.P.M.; Jager, M.J. Soluble HLA in the Aqueous Humour of Uveal Melanoma Is Associated with Unfavourable Tumour Characteristics. Cancers 2019, 11, 1202. https://doi.org/10.3390/cancers11081202

AMA Style

Wierenga APA, Gezgin G, van Beelen E, Eikmans M, Spruyt-Gerritse M, Brouwer NJ, Versluis M, Verdijk RM, van Duinen SG, Marinkovic M, Luyten GPM, Jager MJ. Soluble HLA in the Aqueous Humour of Uveal Melanoma Is Associated with Unfavourable Tumour Characteristics. Cancers. 2019; 11(8):1202. https://doi.org/10.3390/cancers11081202

Chicago/Turabian Style

Wierenga, Annemijn P.A., Gülçin Gezgin, Els van Beelen, Michael Eikmans, Marijke Spruyt-Gerritse, Niels J. Brouwer, Mieke Versluis, Robert M. Verdijk, Sjoerd G. van Duinen, Marina Marinkovic, Gregorius P.M. Luyten, and Martine J. Jager. 2019. "Soluble HLA in the Aqueous Humour of Uveal Melanoma Is Associated with Unfavourable Tumour Characteristics" Cancers 11, no. 8: 1202. https://doi.org/10.3390/cancers11081202

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