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Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes

1
La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne 3086, Australia
2
Olivia Newton-John Cancer Research Institute, Level 5, ONJ Centre, Heidelberg Vic 3084, Australia
3
School of Cancer Medicine, La Trobe University, Melbourne 3086, Australia
4
Garvan Institute of Medical Research, St Vincent’s Clinical School, Darlinghurst, NSW 2010, Australia
5
Consultant Pathologist, Anatpath. 120 Gardenvale Rd, Gardenvale Melbourne 3185, Australia
6
CancerProbe Pty Ltd, PO Box 2237, Prahran 3181, Australia
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Cancers 2019, 11(5), 682; https://doi.org/10.3390/cancers11050682
Received: 17 April 2019 / Revised: 8 May 2019 / Accepted: 13 May 2019 / Published: 16 May 2019
PDF [1899 KB, uploaded 16 May 2019]
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Abstract

Tumor antigens are responsible for initiating an immune response in cancer patients, and their identification may provide new biomarkers for cancer diagnosis and targets for immunotherapy. The general use of serum antibodies to identify tumor antigens has several drawbacks, including dilution, complex formation, and background reactivity. In this study, antibodies were generated from antibody-secreting cells (ASC) present in tumor-draining lymph nodes of 20 breast cancer patients (ASC-probes) and were used to screen breast cancer cell lines and protein microarrays. Half of the ASC-probes reacted strongly against extracts of the MCF-7 breast cancer cell line, but each with a distinct antigen recognition profile. Three of the positive ASC-probes reacted differentially with recombinant antigens on a microarray containing cancer-related proteins. The results of this study show that lymph node-derived ASC-probes provide a highly specific source of tumor-specific antibodies. Each breast cancer patient reacts with a different antibody profile which indicates that targeted immunotherapies may need to be personalized for individual patients. Focused microarrays in combination with ASC-probes may be useful in providing immune profiles and identifying tumor antigens of individual cancer patients.
Keywords: tumor antigen; immune profile; lymph node; antibody secreting cell; biomarker; microarray; breast cancer; immunotherapy tumor antigen; immune profile; lymph node; antibody secreting cell; biomarker; microarray; breast cancer; immunotherapy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Young, A.R.; Duarte, J.D.G.; Coulson, R.; O’Brien, M.; Deb, S.; Lopata, A.; Behren, A.; Mathivanan, S.; Lim, E.; Meeusen, E. Immunoprofiling of Breast Cancer Antigens Using Antibodies Derived from Local Lymph Nodes. Cancers 2019, 11, 682.

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