Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA): A Potential New Tool for Early Detection of Ovarian Cancer
AbstractObjectives: To conduct a comprehensive glycopeptide spectra analysis of serum between cancer and non-cancer patients to identify early biomarkers of epithelial ovarian cancer (EOC). Methods: Approximately 30,000 glycopeptide peaks were detected from the digested serum glycoproteins of 39 EOC patients (23 early-stage, 16 advanced-stage) and 45 non-cancer patients (27 leiomyoma and ovarian cyst cases, 18 endometrioma cases) by liquid chromatography mass spectrometry (LC–MS). The differential glycopeptide peak spectra were analyzed to distinguish between cancer and non-cancer groups by employing multivariate analysis including principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and heat maps. Results: Examined spectral peaks were filtered down to 2281 serum quantitative glycopeptide signatures for differentiation between ovarian cancer and controls using multivariate analysis. The OPLS-DA model using cross-validation parameters R2 and Q2 and score plots of the serum samples significantly differentiated the EOC group from the non-cancer control group. In addition, women with early-stage clear cell carcinoma and endometriomas were clearly distinguished from each other by OPLS-DA as well as by PCA and heat maps. Conclusions: Our study demonstrates the potential of comprehensive serum glycoprotein analysis as a useful tool for ovarian cancer detection. View Full-Text
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Hayashi, M.; Matsuo, K.; Tanabe, K.; Ikeda, M.; Miyazawa, M.; Yasaka, M.; Machida, H.; Shida, M.; Imanishi, T.; Grubbs, B.H.; Hirasawa, T.; Mikami, M. Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA): A Potential New Tool for Early Detection of Ovarian Cancer. Cancers 2019, 11, 591.
Hayashi M, Matsuo K, Tanabe K, Ikeda M, Miyazawa M, Yasaka M, Machida H, Shida M, Imanishi T, Grubbs BH, Hirasawa T, Mikami M. Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA): A Potential New Tool for Early Detection of Ovarian Cancer. Cancers. 2019; 11(5):591.Chicago/Turabian Style
Hayashi, Masaru; Matsuo, Koji; Tanabe, Kazuhiro; Ikeda, Masae; Miyazawa, Mariko; Yasaka, Miwa; Machida, Hiroko; Shida, Masako; Imanishi, Tadashi; Grubbs, Brendan H.; Hirasawa, Takeshi; Mikami, Mikio. 2019. "Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA): A Potential New Tool for Early Detection of Ovarian Cancer." Cancers 11, no. 5: 591.
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