Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine
Abstract5-Aminolevulinic acid (5-ALA), a commonly used photosensitizer in photodynamic detection (PDD) and therapy (PDT), is converted in situ to the established photosensitizer protoporphyrin IX (PpIX) via the heme biosynthetic pathway. To extend 5-ALA-PDT application, we evaluated the PpIX fluorescence induced by exogenous 5-ALA in various veterinary tumors and treated canine and feline tumors. 5-ALA-PDD sensitivity and specificity in the whole sample group for dogs and cats combined were 89.5 and 50%, respectively. Notably, some small tumors disappeared upon 5-ALA-PDT. Although single PDT application was not curative, repeated PDT+/−chemotherapy achieved long-term tumor control. We analyzed the relationship between intracellular PpIX concentration and 5-ALA-PDT in vitro cytotoxicity using various primary tumor cells and determined the correlation between intracellular PpIX concentration and 5-ALA transporter and metabolic enzyme mRNA expression levels. 5-ALA-PDT cytotoxicity in vitro correlated with intracellular PpIX concentration in carcinomas. Ferrochelatase mRNA expression levels strongly negatively correlated with PpIX accumulation, representing the first report of a correlation between mRNA expression related to PpIX accumulation and PpIX concentration in canine tumor cells. Our findings suggested that the results of 5-ALA-PDD might be predictive for 5-ALA-PDT therapeutic effects for carcinomas, with 5-ALA-PDT plus chemotherapy potentially increasing the probability of tumor control in veterinary medicine. View Full-Text
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Osaki, T.; Yokoe, I.; Sunden, Y.; Ota, U.; Ichikawa, T.; Imazato, H.; Ishii, T.; Takahashi, K.; Ishizuka, M.; Tanaka, T.; Li, L.; Yamashita, M.; Murahata, Y.; Tsuka, T.; Azuma, K.; Ito, N.; Imagawa, T.; Okamoto, Y. Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine. Cancers 2019, 11, 495.
Osaki T, Yokoe I, Sunden Y, Ota U, Ichikawa T, Imazato H, Ishii T, Takahashi K, Ishizuka M, Tanaka T, Li L, Yamashita M, Murahata Y, Tsuka T, Azuma K, Ito N, Imagawa T, Okamoto Y. Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine. Cancers. 2019; 11(4):495.Chicago/Turabian Style
Osaki, Tomohiro; Yokoe, Inoru; Sunden, Yuji; Ota, Urara; Ichikawa, Tomoki; Imazato, Hideo; Ishii, Takuya; Takahashi, Kiwamu; Ishizuka, Masahiro; Tanaka, Tohru; Li, Liming; Yamashita, Masamichi; Murahata, Yusuke; Tsuka, Takeshi; Azuma, Kazuo; Ito, Norihiko; Imagawa, Tomohiro; Okamoto, Yoshiharu. 2019. "Efficacy of 5-Aminolevulinic Acid in Photodynamic Detection and Photodynamic Therapy in Veterinary Medicine." Cancers 11, no. 4: 495.
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