Radiosensitivity of Cancer Cells Is Regulated by Translationally Controlled Tumor Protein
Abstract
:1. Introduction
2. Results
2.1. TCTP Expression Inversely Correlates with Radiosensitivity of Cancer Cells
2.2. TCTP Is Involved in Radioresistance of Cancer Cells
2.3. TCTP Knockdown Potentiates Radiation-Induced DNA Damage
2.4. p53 Is Involved in TCTP-Mediated Radioresistance of A549 Cells
2.5. TCTP Knockdown Enhances Radiosensitivity in Tumor Xenograft Mouse Model
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Culture Conditions
4.2. Antibodies
4.3. Clonogenic Formation Assays
4.4. Irradiation
4.5. Transfection
4.6. Generation of Stable Cell Lines by Lentiviral Transduction
4.7. Immunoblotting
4.8. Annexin V PI Apoptosis Assay
4.9. Comet Assay
4.10. Cell Cycle Analysis
4.11. Experimental Animals
4.12. Xenograft
4.13. Immunohistochemistry
4.14. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Jung, J.; Lee, J.-S.; Lee, Y.-S.; Lee, K. Radiosensitivity of Cancer Cells Is Regulated by Translationally Controlled Tumor Protein. Cancers 2019, 11, 386. https://doi.org/10.3390/cancers11030386
Jung J, Lee J-S, Lee Y-S, Lee K. Radiosensitivity of Cancer Cells Is Regulated by Translationally Controlled Tumor Protein. Cancers. 2019; 11(3):386. https://doi.org/10.3390/cancers11030386
Chicago/Turabian StyleJung, Jiwon, Ji-Sun Lee, Yun-Sil Lee, and Kyunglim Lee. 2019. "Radiosensitivity of Cancer Cells Is Regulated by Translationally Controlled Tumor Protein" Cancers 11, no. 3: 386. https://doi.org/10.3390/cancers11030386