Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood
AbstractWe selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients. View Full-Text
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Zamay, G.S.; Kolovskaya, O.S.; Ivanchenko, T.I.; Zamay, T.N.; Veprintsev, D.V.; Grigorieva, V.L.; Garanzha, I.I.; Krat, A.V.; Glazyrin, Y.E.; Gargaun, A.; Lapin, I.N.; Svetlichnyi, V.A.; Berezovski, M.V.; Kichkailo, A.S. Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood. Cancers 2019, 11, 351.
Zamay GS, Kolovskaya OS, Ivanchenko TI, Zamay TN, Veprintsev DV, Grigorieva VL, Garanzha II, Krat AV, Glazyrin YE, Gargaun A, Lapin IN, Svetlichnyi VA, Berezovski MV, Kichkailo AS. Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood. Cancers. 2019; 11(3):351.Chicago/Turabian Style
Zamay, Galina S.; Kolovskaya, Olga S.; Ivanchenko, Tatiana I.; Zamay, Tatiana N.; Veprintsev, Dmitry V.; Grigorieva, Valentina L.; Garanzha, Irina I.; Krat, Alexey V.; Glazyrin, Yury E.; Gargaun, Ana; Lapin, Ivan N.; Svetlichnyi, Valery A.; Berezovski, Maxim V.; Kichkailo, Anna S. 2019. "Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood." Cancers 11, no. 3: 351.
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