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Article

Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device

1
Department of Electronic Engineering, Hanyang University College of Engineering, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea
2
Department of Life Science and Research Institute for Natural Sciences, Hanyang University College of Natural Sciences, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea
3
Institute of Nano Science and Technology, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea
4
Department of Internal Medicine, Hanyang University College of Medicine, Hanyang University Medical Center, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Korea
*
Author to whom correspondence should be addressed.
Cancers 2019, 11(2), 200; https://doi.org/10.3390/cancers11020200
Received: 19 December 2018 / Revised: 24 January 2019 / Accepted: 1 February 2019 / Published: 10 February 2019
(This article belongs to the Special Issue Liquid Biopsy for Cancer)
The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterogeneity. Here, we present a slanted weir microfluidic device that utilizes the size and deformability of CTCs to separate them from the unprocessed whole blood. By testing its ability using a highly invasive breast cancer cell line, our device achieved a 97% separation efficiency, while showing an 8-log depletion of erythrocytes and 5.6-log depletion of leukocytes. We also developed an image analysis tool that was able to characterize the various morphologies and differing deformability of the separating cells. From the results, we believe our system possesses a high potential for liquid biopsy, aiding future cancer research. View Full-Text
Keywords: circulating tumor cell; cell separation; microfluidics circulating tumor cell; cell separation; microfluidics
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MDPI and ACS Style

Yoon, Y.; Lee, J.; Ra, M.; Gwon, H.; Lee, S.; Kim, M.Y.; Yoo, K.-C.; Sul, O.; Kim, C.G.; Kim, W.-Y.; Park, J.-G.; Lee, S.-J.; Lee, Y.Y.; Choi, H.S.; Lee, S.-B. Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device. Cancers 2019, 11, 200. https://doi.org/10.3390/cancers11020200

AMA Style

Yoon Y, Lee J, Ra M, Gwon H, Lee S, Kim MY, Yoo K-C, Sul O, Kim CG, Kim W-Y, Park J-G, Lee S-J, Lee YY, Choi HS, Lee S-B. Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device. Cancers. 2019; 11(2):200. https://doi.org/10.3390/cancers11020200

Chicago/Turabian Style

Yoon, Yousang, Jusin Lee, Moonsoo Ra, Hyeokshin Gwon, Seungwon Lee, Min Y. Kim, Ki-Chun Yoo, Onejae Sul, Chul G. Kim, Whoi-Yul Kim, Jea-Gun Park, Su-Jae Lee, Young Y. Lee, Ho S. Choi, and Seung-Beck Lee. 2019. "Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device" Cancers 11, no. 2: 200. https://doi.org/10.3390/cancers11020200

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