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Gathering Novel Circulating Exosomal microRNA in Osteosarcoma Cell Lines and Possible Implications for the Disease

1
IRCCS ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad alta specializzazione), Research Department, 90127 Palermo, Italy
2
IRCCS ISTITUTO ORTOPEDICO RIZZOLI, 40136 Bologna, Italy
3
Fondazione Ri.MED, 90133 Palermo, Italy
4
Body Fluids Biobank, Clinical Pathology, IRCCS Regina Elena Cancer National Institute, 00100 Rome, Italy
5
Dept. of Orthopaedic Oncology, IRCCS Regina Elena National Cancer Institute, 00100 Rome, Italy
*
Author to whom correspondence should be addressed.
The authors contributed equally to the study.
Cancers 2019, 11(12), 1924; https://doi.org/10.3390/cancers11121924
Received: 16 October 2019 / Revised: 26 November 2019 / Accepted: 28 November 2019 / Published: 3 December 2019
(This article belongs to the Special Issue Role of miRNAs in Cancer—Analysis of Their Targetome)
One of the goals of personalized medicine is to understand and treat diseases with greater precision through the molecular profile of the patient. This profiling is becoming a powerful tool for the discovery of novel biomarkers that can guide physicians in assessing, in advance, the disease stage, and monitoring disease progression. Circulating miRNAs and exosomal miRNAs, a group of small non-coding RNAs, are considered the gold standard diagnostic biomarkers for human diseases. We have previously demonstrated that osteosarcoma-derived exosomes are able to influence crucial mechanisms inside tumor niches, inducing osteoclast differentiation, and sustaining bone resorption activity. Here we discovered, through Next-Generation Sequencing (NGS), eight novel microRNAs in three different osteosarcoma cell lines, and assessed the selective packaging into the exosomes released. We then investigated, as proof-of-principle, the presence of the novel microRNAs in osteosarcoma patient samples, and found that 5 of the 8 novel microRNAs were more present in circulating exosomes of osteosarcoma patients compared with the controls. These results raise a question: Could the 8 novel microRNAs play a role for osteosarcoma pathogenesis? Although still premature, the results are encouraging, and further studies with a validation in a larger cohort are needed. View Full-Text
Keywords: microRNAs; exosomes; liquid biopsy microRNAs; exosomes; liquid biopsy
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Cuscino, N.; Raimondi, L.; De Luca, A.; Carcione, C.; Russelli, G.; Conti, L.; Baldi, J.; Conaldi, P.G.; Giavaresi, G.; Gallo, A. Gathering Novel Circulating Exosomal microRNA in Osteosarcoma Cell Lines and Possible Implications for the Disease. Cancers 2019, 11, 1924.

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