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Article

Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment

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Department of Radiology and Physical Medicine, University of Granada, 18016 Granada, Spain
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Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, 18100 Granada, Spain
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Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, 18016 Granada, Spain
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Biosanitary Research Institute, ibs.Granada, 18012 Granada, Spain
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Department of Health Sciences, University of Jaén, E-23071 Jaén, Spain
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CIBER Epidemiology and Public Health (CIBERESP), 28029 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Cancers 2019, 11(11), 1781; https://doi.org/10.3390/cancers11111781
Received: 4 October 2019 / Revised: 31 October 2019 / Accepted: 8 November 2019 / Published: 12 November 2019
Breast cancer is the most common cancer in women. Radiotherapy (RT) is one of the mainstay treatments for cancer but in some cases is not effective. Cancer stem cells (CSCs) within the tumor can be responsible for recurrence and metastasis after RT. Matrix metalloproteases (MMPs), regulated mainly by tissue inhibitors of metalloproteinases (TIMPs) and histone deacetylases (HDACs), may also contribute to tumor development by modifying its activity after RT. The aim of this work was to study the effects of RT on the expression of MMPs, TIMPs and HDACs on different cell subpopulations in MCF-7, MDA-MB-231 and SK-BR-3 cell lines. We assessed the in vitro expression of these genes in different 3D culture models and induced tumors in female NSG mice by orthotopic xenotransplants. Our results showed that gene expression is related to the cell subpopulation studied, the culture model used and the single radiation dose administered. Moreover, the crucial role played by the microenvironment in terms of cell interactions and CSC plasticity in tumor growth and RT outcome is also shown, supporting the use of higher doses (6 Gy) to achieve better control of tumor development. View Full-Text
Keywords: breast cancer outcomes; radiotherapy; cancer stem cells; stemness phenotype; irradiation; 3D culture; matrix metalloproteases; inhibitors; targeted treatment; Matrigel breast cancer outcomes; radiotherapy; cancer stem cells; stemness phenotype; irradiation; 3D culture; matrix metalloproteases; inhibitors; targeted treatment; Matrigel
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MDPI and ACS Style

Olivares-Urbano, M.A.; Griñán-Lisón, C.; Ríos-Arrabal, S.; Artacho-Cordón, F.; Torralbo, A.I.; López-Ruiz, E.; Marchal, J.A.; Núñez, M.I. Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment. Cancers 2019, 11, 1781. https://doi.org/10.3390/cancers11111781

AMA Style

Olivares-Urbano MA, Griñán-Lisón C, Ríos-Arrabal S, Artacho-Cordón F, Torralbo AI, López-Ruiz E, Marchal JA, Núñez MI. Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment. Cancers. 2019; 11(11):1781. https://doi.org/10.3390/cancers11111781

Chicago/Turabian Style

Olivares-Urbano, María A., Carmen Griñán-Lisón, Sandra Ríos-Arrabal, Francisco Artacho-Cordón, Ana I. Torralbo, Elena López-Ruiz, Juan A. Marchal, and María I. Núñez 2019. "Radiation and Stemness Phenotype May Influence Individual Breast Cancer Outcomes: The Crucial Role of MMPs and Microenvironment" Cancers 11, no. 11: 1781. https://doi.org/10.3390/cancers11111781

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