Tumor and Cerebrospinal Fluid microRNAs in Primary Central Nervous System Lymphomas
Abstract
:1. Introduction
2. Results
2.1. miR-155, miR-196b, miR-9, miR-125b, and miR-Let-7b Levels in FFPET Brain Biopsies and CSF Samples from Patients with CNS, DLBCL vs. n-ML
2.2. miR-21, miR-19b, and miR-92a Levels in CSFs and Brain Biopsies from Patients with CNS DLBCL vs. n-ML
2.3. Survival Analyses
2.4. CNS DLBCL Immunophenotypes and miR Expression
3. Discussion
4. Materials and Methods
4.1. Patients and Samples
4.1.1. Patients
4.1.2. Sample Collection
4.1.3. Diagnostic Procedures: Immunohistochemical Staining of FFPET Samples
4.1.4. Diagnostic Procedures: Immunophenotyping, Morphology and Proliferation Evaluation of CSF Cells
4.1.5. Final Diagnosis
4.2. RNA Extraction and RT-qPCR
4.3. Statistical Analyses
5. Conclusions
- Tumor miR-155, miR-196b, miR-9, miR-125b, and miR-let-7b expression levels are significantly different in PCNSL and in non-malignant brain lesions.
- A logistic regression model is proposed to discriminate between PCNSL and non-malignant brain lesions.
- We confirm the value of cerebrospinal fluid miR-21, miR-19b, and miR-92a profiles as potential CNS DLBCL markers.
- PCNSL CSFs and the relevant biopsy samples are characterized by specific, different microRNA profiles.
- The examined microRNA profiles do not influence overall survival of PCNSL patients.
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zajdel, M.; Rymkiewicz, G.; Sromek, M.; Cieslikowska, M.; Swoboda, P.; Kulinczak, M.; Goryca, K.; Bystydzienski, Z.; Blachnio, K.; Ostrowska, B.; et al. Tumor and Cerebrospinal Fluid microRNAs in Primary Central Nervous System Lymphomas. Cancers 2019, 11, 1647. https://doi.org/10.3390/cancers11111647
Zajdel M, Rymkiewicz G, Sromek M, Cieslikowska M, Swoboda P, Kulinczak M, Goryca K, Bystydzienski Z, Blachnio K, Ostrowska B, et al. Tumor and Cerebrospinal Fluid microRNAs in Primary Central Nervous System Lymphomas. Cancers. 2019; 11(11):1647. https://doi.org/10.3390/cancers11111647
Chicago/Turabian StyleZajdel, Michalina, Grzegorz Rymkiewicz, Maria Sromek, Maria Cieslikowska, Pawel Swoboda, Mariusz Kulinczak, Krzysztof Goryca, Zbigniew Bystydzienski, Katarzyna Blachnio, Beata Ostrowska, and et al. 2019. "Tumor and Cerebrospinal Fluid microRNAs in Primary Central Nervous System Lymphomas" Cancers 11, no. 11: 1647. https://doi.org/10.3390/cancers11111647