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Adenosine Triphosphate Measurement in Deep Sea Using a Microfluidic Device

1
Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima–cho, Yokosuka, Kanagawa 237-0061, Japan
2
Research and Education Faculty, Kochi University, B200 Monobe, Nankoku, Kochi 783-8502, Japan
3
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro–ku, 153-8505 Tokyo, Japan
*
Author to whom correspondence should be addressed.
Micromachines 2018, 9(8), 370; https://doi.org/10.3390/mi9080370
Received: 10 May 2018 / Revised: 21 July 2018 / Accepted: 24 July 2018 / Published: 27 July 2018
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Abstract

Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L–L (luciferin–luciferase) assay with a miniature pumping unit and a photometry module for the measurement of the bioluminescence intensity. A heater and a temperature sensor were also included in the system to maintain an optimal temperature for the L–L reaction. In this study, the analyzer was evaluated in deep sea environments, reaching a depth of 200 m using a remotely operated underwater vehicle. We show that the ATP analyzer successfully operated in the deep-sea environment and accurately quantified total ATP within the concentration lower than 5 × 10−11 M. View Full-Text
Keywords: ATP; microfluidic device; luciferin–luciferase assay ATP; microfluidic device; luciferin–luciferase assay
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Fukuba, T.; Noguchi, T.; Okamura, K.; Fujii, T. Adenosine Triphosphate Measurement in Deep Sea Using a Microfluidic Device. Micromachines 2018, 9, 370.

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