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Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals

1
Department of Chemistry, Lomonosov Moscow State University, Leninskiye Gory 1-3, 119991 Moscow, Russia
2
Federal Research and Clinical Centre of Physical-Chemical Medicine, Malaya Pirogovskaya, 1A, 119992 Moscow, Russia
3
Nottingham Trent University, School of Science and Technology, Clifton Lane, Nottingham NG11 8NS, UK
*
Author to whom correspondence should be addressed.
Micromachines 2018, 9(6), 307; https://doi.org/10.3390/mi9060307
Received: 9 May 2018 / Revised: 1 June 2018 / Accepted: 11 June 2018 / Published: 19 June 2018
(This article belongs to the Special Issue Self-Assembly of Polymers)
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Abstract

Porous vaterite crystals of CaCO3 are extensively used for the fabrication of self-assembled polymer-based microparticles (capsules, beads, etc.) utilized for drug delivery and controlled release. The nature of the polymer used plays a crucial role and discovery of new perspective biopolymers is essential to assemble microparticles with desired characteristics, such as biocompatibility, drug loading efficiency/capacity, release rate, and stability. Glycoprotein mucin is tested here as a good candidate to assemble the microparticles because of high charge due to sialic acids, mucoadhesive properties, and a tendency to self-assemble, forming gels. Mucin loading into the crystals via co-synthesis is twice as effective as via adsorption into preformed crystals. Desialylated mucin has weaker binding to the crystals most probably due to electrostatic interactions between sialic acids and calcium ions on the crystal surface. Improved loading of low-molecular-weight inhibitor aprotinin into the mucin-containing crystals is demonstrated. Multilayer capsules (mucin/protamine)3 have been made by the layer-by-layer self-assembly. Interestingly, the deposition of single mucin layers (mucin/water)3 has also been proven, however, the capsules were unstable, most probably due to additional (to hydrogen bonding) electrostatic interactions in the case of the two polymers used. Finally, approaches to load biologically-active compounds (BACs) into the mucin-containing microparticles are discussed. View Full-Text
Keywords: CaCO3; mucin; adsorption; co-synthesis; layer-by-layer; protamine; aprotinin CaCO3; mucin; adsorption; co-synthesis; layer-by-layer; protamine; aprotinin
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Balabushevich, N.G.; Sholina, E.A.; Mikhalchik, E.V.; Filatova, L.Y.; Vikulina, A.S.; Volodkin, D. Self-Assembled Mucin-Containing Microcarriers via Hard Templating on CaCO3 Crystals. Micromachines 2018, 9, 307.

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