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Determination of the Three-Dimensional Rate of Cancer Cell Rotation in an Optically-Induced Electrokinetics Chip Using an Optical Flow Algorithm

1
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao 066004, China
2
Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong 999077, China
3
Shenzhen Academy of Robotics, Shenzhen 518000, China
*
Authors to whom correspondence should be addressed.
Micromachines 2018, 9(3), 118; https://doi.org/10.3390/mi9030118
Received: 28 January 2018 / Revised: 21 February 2018 / Accepted: 1 March 2018 / Published: 8 March 2018
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Abstract

Our group has reported that Melan-A cells and lymphocytes undergo self-rotation in a homogeneous AC electric field, and found that the rotation velocity of these cells is a key indicator to characterize their physical properties. However, the determination of the rotation properties of a cell by human eyes is both gruesome and time consuming, and not always accurate. In this paper, a method is presented to more accurately determine the 3D cell rotation velocity and axis from a 2D image sequence captured by a single camera. Using the optical flow method, we obtained the 2D motion field data from the image sequence and back-project it onto a 3D sphere model, and then the rotation axis and velocity of the cell were calculated. After testing the algorithm on animated image sequences, experiments were also performed on image sequences of real rotating cells. All of these results indicate that this method is accurate, practical, and useful. Furthermore, the method presented there can also be used to determine the 3D rotation velocity of other types of spherical objects that are commonly used in microfluidic applications, such as beads and microparticles. View Full-Text
Keywords: cell self-rotation; single cell rotation; optically induced electrokinetics; optical flow algorithm cell self-rotation; single cell rotation; optically induced electrokinetics; optical flow algorithm
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Zhao, Y.; Jia, D.; Sha, X.; Zhang, G.; Li, W.J. Determination of the Three-Dimensional Rate of Cancer Cell Rotation in an Optically-Induced Electrokinetics Chip Using an Optical Flow Algorithm. Micromachines 2018, 9, 118.

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