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Open AccessArticle

Hyaluronate-Functionalized Graphene for Label-Free Electrochemical Cytosensing

1
School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, China
2
Medical College, Henan University of Science and Technology, Luoyang 471023, China
3
School of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467000, China
4
Pingdingshan No. 1 Middle School, Pingdingshan 467000, China
*
Authors to whom correspondence should be addressed.
Micromachines 2018, 9(12), 669; https://doi.org/10.3390/mi9120669
Received: 22 November 2018 / Revised: 12 December 2018 / Accepted: 15 December 2018 / Published: 18 December 2018
(This article belongs to the Special Issue Graphene Nanoelectronic Devices)
Electrochemical sensors for early tumor cell detection are currently an important area of research, as this special region directly improves the efficiency of cancer treatment. Functional graphene is a promising alternative for selective recognition and capture of target cancer cells. In our work, an effective cytosensor of hyaluronate-functionalized graphene (HG) was prepared through chemical reduction of graphene oxide. The as-prepared HG nanostructures were characterized with Fourier transform infrared spectroscopy and transmission electron microscopy coupled with cyclic voltammograms and electrochemical impedance spectroscopy, respectively. The self-assembly of HG with ethylene diamine, followed by sodium hyaluronate, enabled the fabrication of a label-free electrochemical impedance spectroscopy cytosensor with high stability and biocompatibility. Finally, the proposed cytosensor exhibited satisfying electrochemical behavior and cell-capture capacity for human colorectal cancer cells HCT-116, and also displayed a wide linear range, from 5.0 × 102 cells∙mL−1 to 5.0 × 106 cells∙mL−1, and a low detection limit of 100 cells∙mL−1 (S/N = 3) for quantification. This work paves the way for graphene applications in electrochemical cytosensing and other bioassays. View Full-Text
Keywords: biocompatible interface; graphene oxide; colorectal cancer cells HCT-116; electrochemical impedance spectroscopy biocompatible interface; graphene oxide; colorectal cancer cells HCT-116; electrochemical impedance spectroscopy
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MDPI and ACS Style

Jing, A.; Zhang, C.; Liang, G.; Feng, W.; Tian, Z.; Jing, C. Hyaluronate-Functionalized Graphene for Label-Free Electrochemical Cytosensing. Micromachines 2018, 9, 669.

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