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Open AccessFeature PaperArticle

Development of Temperature Control Solutions for Non-Instrumented Nucleic Acid Amplification Tests (NINAAT)

1
Thomas Johann Seebeck Department of Electronics, Tallinn University of Technology, Ehitajate Tee 5, 12616 Tallinn, Estonia
2
Selfdiagnostics Deutschland GmbH, 04103 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Nam-Trung Nguyen, Stephen Haswell, Yi Heng Nai and Kirsty Shaw
Micromachines 2017, 8(6), 180; https://doi.org/10.3390/mi8060180
Received: 24 April 2017 / Revised: 23 May 2017 / Accepted: 1 June 2017 / Published: 7 June 2017
(This article belongs to the Special Issue Application of Microfluidic Methodology for the Analysis of DNA)
Non-instrumented nucleic acid amplification tests (NINAAT) are a novel paradigm in portable molecular diagnostics. They offer the high detection accuracy characteristic of nucleic acid amplification tests (NAAT) in a self-contained device, without the need for any external instrumentation. These Point-of-Care tests typically employ a Lab-on-a-Chip for liquid handling functionality, and perform isothermal nucleic acid amplification protocols that require low power but high accuracy temperature control in a single well-defined temperature range. We propose temperature control solutions based on commercially available heating elements capable of meeting these challenges, as well as demonstrate the process by which such elements can be fitted to a NINAAT system. Self-regulated and thermostat-controlled resistive heating elements were evaluated through experimental characterization as well as thermal analysis using the finite element method (FEM). We demonstrate that the proposed solutions can support various NAAT protocols, as well as demonstrate an optimal solution for the loop-mediated isothermal amplification (LAMP) protocol. Furthermore, we present an Arduino-compatible open-source thermostat developed for NINAAT applications. View Full-Text
Keywords: lab-on-a-chip; LoC; finite element modelling; resistive heating; Point-of-Care; PoC; temperature control; computer aided design; microfluidics; isothermal nucleic acid amplification tests; NINAAT; NAAT lab-on-a-chip; LoC; finite element modelling; resistive heating; Point-of-Care; PoC; temperature control; computer aided design; microfluidics; isothermal nucleic acid amplification tests; NINAAT; NAAT
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MDPI and ACS Style

Pardy, T.; Rang, T.; Tulp, I. Development of Temperature Control Solutions for Non-Instrumented Nucleic Acid Amplification Tests (NINAAT). Micromachines 2017, 8, 180.

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