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Micromachines 2017, 8(4), 104;

Paper-Based Colorimetric Biosensor for Tear Glucose Measurements

Instituto de Química, Universidade Federal de Goiás, 74690-900 Goiânia, GO, Brazil
Faculdade de Farmácia, Universidade Federal de Goiás, 74605-170 Goiânia, GO, Brazil
Instituto Nacional de Ciência e Tecnologia em Bioanalítica (INCTBio), 13083-970 Campinas, SP, Brazil
Author to whom correspondence should be addressed.
Academic Editors: Frank A. Gomez and Carlos D. Garcia
Received: 8 February 2017 / Revised: 15 March 2017 / Accepted: 22 March 2017 / Published: 29 March 2017
(This article belongs to the Special Issue Paper Microfluidics: Fundamental Studies and Applications)
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This paper describes a paper-based colorimetric biosensor for measuring glucose concentration levels in human tear samples. Colorimetric biosensors were wax printed on paper platforms and modified with chitosan previously prepared in acetic acid. The proposed device was explored to measure the glucose levels in human tear samples using 3,3′,5,5′-tetramethylbenzydine (TMB) as the chromogenic reagent. The paper-based colorimetric biosensor exhibited a linear behavior for the glucose concentration range between 0.1 and 1.0 mM. The achieved analytical sensitivity and limit of detection (LOD) were 84 AU/mM and 50 µM, respectively. Moreover, the device provided analytical reliability and no statistical difference when compared to the data recorded with a commercial glucometer. The proof-of-concept of our device was successfully demonstrated by measuring the glucose levels in six tear samples from nondiabetic subjects. In general, the results showed that the colorimetric biosensor has noticeable potential to be used as a powerful tool for tear glucose monitoring, since this fluid offers lower potential interferences, non-invasive sample collection and is pain-free. Furthermore, the proposed device could facilitate the treatment of diabetic patients who need constant control of glucose levels and cannot tolerate multiple finger sticks per day. View Full-Text
Keywords: bioanalytical chemistry; clinical diagnostics; glucose enzymatic reaction; non-invasive diagnostics; paper microfluidics; point-of-care testing bioanalytical chemistry; clinical diagnostics; glucose enzymatic reaction; non-invasive diagnostics; paper microfluidics; point-of-care testing

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Gabriel, E.F.M.; Garcia, P.T.; Lopes, F.M.; Coltro, W.K.T. Paper-Based Colorimetric Biosensor for Tear Glucose Measurements. Micromachines 2017, 8, 104.

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