The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel

Yan, Sheng; Yuan, Dan; Zhao, Qianbin; Zhang, Jun; Li, Weihua

doi:10.3390/mi8110315

Open AccessArticle

The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel

by

Sheng Yan

^1,*,

Dan Yuan

¹,

Qianbin Zhao

¹,

Jun Zhang

²

and

Weihua Li

^1,*

¹

School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong, NSW 2522, Australia

²

School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China

^*

Authors to whom correspondence should be addressed.

Micromachines 2017, 8(11), 315; https://doi.org/10.3390/mi8110315

Submission received: 20 September 2017 / Revised: 20 October 2017 / Accepted: 23 October 2017 / Published: 25 October 2017

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Abstract

In the capillary venules, blood cells auto-separate with red blood cells aggregating near the centre of vessel and the nucleated cells marginating toward the wall of vessel. In this experiment, we used cell margination to help enrich the Jurkat cells via a groove-based channel which provides a vertical expansion-contraction structure, wherein the red blood cells invade the grooves and push the Jurkat cells to the bottom of the channel. The secondary flows induced by the anisotropic grooves bring the Jurkat cells to the right sidewall. Rigid, 13-µm diameter polystyrene particles were spiked into the whole blood to verify the operating principle under various working conditions, and then tests were carried out using Jurkat cells (~15 µm). The performance of this device was quantified by analysing the cell distribution in a transverse direction at the outlet, and then measuring the cell concentration from the corresponding outlets. The results indicate that Jurkat cells were enriched by 22.3-fold with a recovery rate of 83.4%, thus proving that this microfluidic platform provides a gentle and passive way to isolate intact and viable Jurkat cells.

Keywords: microfluidics; groove-based channel; cell margination; cancer cell

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MDPI and ACS Style

Yan, S.; Yuan, D.; Zhao, Q.; Zhang, J.; Li, W. The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel. Micromachines 2017, 8, 315. https://doi.org/10.3390/mi8110315

AMA Style

Yan S, Yuan D, Zhao Q, Zhang J, Li W. The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel. Micromachines. 2017; 8(11):315. https://doi.org/10.3390/mi8110315

Chicago/Turabian Style

Yan, Sheng, Dan Yuan, Qianbin Zhao, Jun Zhang, and Weihua Li. 2017. "The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel" Micromachines 8, no. 11: 315. https://doi.org/10.3390/mi8110315

APA Style

Yan, S., Yuan, D., Zhao, Q., Zhang, J., & Li, W. (2017). The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel. Micromachines, 8(11), 315. https://doi.org/10.3390/mi8110315

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The Continuous Concentration of Particles and Cancer Cell Line Using Cell Margination in a Groove-Based Channel

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