Next Article in Journal
Solvent-Free Patterning of Colloidal Quantum Dot Films Utilizing Shape Memory Polymers
Next Article in Special Issue
Electrophoretic Concentration and Electrical Lysis of Bacteria in a Microfluidic Device Using a Nanoporous Membrane
Previous Article in Journal
Complete Procedure for Fabrication of a Fused Silica Ultrarapid Microfluidic Mixer Used in Biophysical Measurements
Previous Article in Special Issue
Combining Electro-Osmotic Flow and FTA® Paper for DNA Analysis on Microfluidic Devices
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessCommunication
Micromachines 2017, 8(1), 17;

Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf

Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA
TeGrex Technologies, Charlottesville, VA 22910, USA
Department of Mechanical and Aerospace Engineering, University of Virginia, Charlottesville, VA 22904, USA
Department of Pathology, University of Virginia, Charlottesville, VA 22904, USA
Applied Research Institute, University of Virginia, Charlottesville, VA 22904, USA
Author to whom correspondence should be addressed.
Academic Editors: Stephen Haswell, Yi Heng Nai and Kirsty Shaw
Received: 30 November 2016 / Revised: 4 January 2017 / Accepted: 4 January 2017 / Published: 9 January 2017
(This article belongs to the Special Issue Application of Microfluidic Methodology for the Analysis of DNA)
Full-Text   |   PDF [4028 KB, uploaded 10 January 2017]   |  


In the last decade, the microfluidic community has witnessed an evolution in fabrication methodologies that deviate from using conventional glass and polymer-based materials. A leading example within this group is the print, cut and laminate (PCL) approach, which entails the laser cutting of microfluidic architecture into ink toner-laden polyester sheets, followed by the lamination of these layers for device assembly. Recent success when applying this method to human genetic fingerprinting has highlighted that it is now ripe for the refinements necessary to render it amenable to mass-manufacture. In this communication, we detail those modifications by identifying and implementing a suitable heat-sensitive adhesive (HSA) material to equip the devices with the durability and resilience required for commercialization and fieldwork. Importantly, this augmentation is achieved without sacrificing any of the characteristics which make the PCL approach attractive for prototyping. Exemplary HSA-devices performed DNA extraction, amplification and separation which, when combined, constitute the complete sequence necessary for human profiling and other DNA-based analyses. View Full-Text
Keywords: microfluidic; adhesive; centrifugal; DNA microfluidic; adhesive; centrifugal; DNA

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Birch, C.; DuVall, J.A.; Le Roux, D.; Thompson, B.L.; Tsuei, A.-C.; Li, J.; Nelson, D.A.; Mills, D.L.; Landers, J.P.; Root, B.E. Rapid Fabrication of Electrophoretic Microfluidic Devices from Polyester, Adhesives and Gold Leaf. Micromachines 2017, 8, 17.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top