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Micromachines 2016, 7(9), 161;

Operation of Droplet-Microfluidic Devices with a Lab Centrifuge

Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences (QB3), University of California, San Francisco, CA 94115, USA
Author to whom correspondence should be addressed.
Academic Editors: Andrew J. deMello and Xavier Casadevall i Solvas
Received: 7 June 2016 / Revised: 12 August 2016 / Accepted: 18 August 2016 / Published: 6 September 2016
(This article belongs to the Special Issue Droplet Microfluidics: Techniques and Technologies, Volume II)
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Microfluidic devices are valuable for a variety of biotechnology applications, such as synthesizing biochemical libraries, screening enzymes, and analyzing single cells. However, normally, the devices are controlled using specialized pumps, which require expert knowledge to operate. Here, we demonstrate operation of poly(dimethylsiloxane) devices without pumps. We build a scaffold that holds the device and reagents to be infused in a format that can be inserted into a 50 mL falcon tube and spun in a common lab centrifuge. By controlling the device design and centrifuge spin speed, we infuse the reagents at controlled flow rates. We demonstrate the encapsulation and culture of clonal colonies of red and green Escherichia coli in droplets seeded from single cells. View Full-Text
Keywords: microfluidics; droplets; poly(dimethylsiloxane); lab on chip microfluidics; droplets; poly(dimethylsiloxane); lab on chip

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ahmed, N.; Sukovich, D.; Abate, A.R. Operation of Droplet-Microfluidic Devices with a Lab Centrifuge. Micromachines 2016, 7, 161.

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