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Multiscale and Multimaterial Fabrication: The Challenge Ahead
Open AccessArticle

A Simple Method for Fabrication of Microstructures Using a PDMS Stamp

1
Sensors and MicroActuators Learning Laboratory (SMALL), Department of Electrical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA
2
Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Nam-Trung Nguyen
Micromachines 2016, 7(10), 173; https://doi.org/10.3390/mi7100173
Received: 14 June 2016 / Revised: 3 September 2016 / Accepted: 19 September 2016 / Published: 1 October 2016
(This article belongs to the Special Issue Biomedical Microfluidic Devices)
We report a simple method to fabricate PDMS (polydimethylsiloxane) microwell arrays on glass by using a PDMS stamp to study cell-to-cell adhesion. In the cell-to-cell study, a glass substrate is required since glass has better cell attachment. The microwell arrays are replicated from an SU-8 master mold, and then are transferred to a glass substrate by lifting the PDMS stamp, followed by oxygen plasma bonding of the PDMS stamp on the glass substrate. For the cell-to-cell adhesion, four different types of PDMS arrays (e.g., rectangle, bowtie, wide-rhombus, and rhombus) were designed to vary the cell-to-cell contact length. The transfer success rates of the microwell arrays were measured as a function of both the contact area of the PDMS and the glass substrate and the different ratios between the base polymers and the curing agent. This method of generating the microwell arrays will enable a simple and robust construction of PDMS-based devices for various biological applications. View Full-Text
Keywords: PDMS microwell; PDMS microwell transfer; PDMS stamp; cell-to-cell study PDMS microwell; PDMS microwell transfer; PDMS stamp; cell-to-cell study
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MDPI and ACS Style

Lee, H.; Koh, D.; Xu, L.; Row, S.; Andreadis, S.T.; Oh, K.W. A Simple Method for Fabrication of Microstructures Using a PDMS Stamp. Micromachines 2016, 7, 173.

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