Next Article in Journal
Elasticity of Carrier Fluid: A Key Factor Affecting Mechanical Phenotyping in Deformability Cytometry
Previous Article in Journal
Increasing Optical Path Lengths in Micro-Fluidic Devices Using a Multi-Pass Cell
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Micromachines
Volume 15
Issue 7
10.3390/mi15070821
micromachines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Research on Key Technologies of Dual-Light-Type Photoelectric Colorimetric Method for Phosphate Determination

by
Hongzhuang Guo
1,
Hao Zhang
2,
Tingting Sun
1,
Xin Wang
1,* and
Ping Gong
2,*
1
School of Physics, Changchun University of Science and Technology, Changchun 130022, China
2
School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China
*
Authors to whom correspondence should be addressed.
Micromachines 2024, 15(7), 821; https://doi.org/10.3390/mi15070821
Submission received: 8 May 2024 / Revised: 19 June 2024 / Accepted: 24 June 2024 / Published: 25 June 2024
Browse Figures
Versions Notes

Abstract

Phosphate plays a crucial role in microbial proliferation, and the regulation of the phosphate concentration can modulate the fermentation efficiency. In this study, based on Lambert–Beer’s Law and the selective absorption characteristics of substances under light, a dual-light-type photoelectric colorimetric device for phosphate determination was designed. The device’s main components, such as the excitation light path and incubation stations, were modeled and simulated. The primary performance of the instrument was verified, and comparative experiments with a UV-1780 spectrophotometer were conducted to validate its performance. The experimental results demonstrate that this device exhibits a high degree of linearity with an R2 value of 0.9956 and a repeatability of ≤1.72%. The average temperature rise rate at the incubation stations was measured at 0.44 °C/s, with a temperature uniformity ≤ ±0.1 °C (temperature set at 37.3 °C). Consistently observed trends in the measurement of 23 CHO cell suspensions using the UV-1780 spectrophotometer further validated the accuracy and reliability of the device’s detection results.
Keywords: photoelectric colorimetry; phosphate detection; finite element analysis photoelectric colorimetry; phosphate detection; finite element analysis

Share and Cite

MDPI and ACS Style

Guo, H.; Zhang, H.; Sun, T.; Wang, X.; Gong, P. Research on Key Technologies of Dual-Light-Type Photoelectric Colorimetric Method for Phosphate Determination. Micromachines 2024, 15, 821. https://doi.org/10.3390/mi15070821

AMA Style

Guo H, Zhang H, Sun T, Wang X, Gong P. Research on Key Technologies of Dual-Light-Type Photoelectric Colorimetric Method for Phosphate Determination. Micromachines. 2024; 15(7):821. https://doi.org/10.3390/mi15070821

Chicago/Turabian Style

Guo, Hongzhuang, Hao Zhang, Tingting Sun, Xin Wang, and Ping Gong. 2024. "Research on Key Technologies of Dual-Light-Type Photoelectric Colorimetric Method for Phosphate Determination" Micromachines 15, no. 7: 821. https://doi.org/10.3390/mi15070821

APA Style

Guo, H., Zhang, H., Sun, T., Wang, X., & Gong, P. (2024). Research on Key Technologies of Dual-Light-Type Photoelectric Colorimetric Method for Phosphate Determination. Micromachines, 15(7), 821. https://doi.org/10.3390/mi15070821

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop