SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Computational Modeling
2.3. Design, Fabrication, and Sealing of PMMA Substrates
2.4. Microchannel Passivation and Functionalization
2.5. Experimental Tests for Particles and Coloured Liquids
2.6. Experimental Tests for Cells
3. Results and Discussion
3.1. Verification of the Numerical Model
3.2. Design and Fabrication of the LOC Devices
3.3. Mixing and Gradient Generation Experiments
3.4. CTC Capture Experiments
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Repetition Rate (RR) | Pulse Energy | Scan Speed | Hatch Distance |
---|---|---|---|
50 kHz | 12 μJ | 40 mm·s–1 | 5 μm |
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Zoupanou, S.; Volpe, A.; Primiceri, E.; Gaudiuso, C.; Ancona, A.; Ferrara, F.; Chiriacò, M.S. SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis. Micromachines 2021, 12, 885. https://doi.org/10.3390/mi12080885
Zoupanou S, Volpe A, Primiceri E, Gaudiuso C, Ancona A, Ferrara F, Chiriacò MS. SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis. Micromachines. 2021; 12(8):885. https://doi.org/10.3390/mi12080885
Chicago/Turabian StyleZoupanou, Sofia, Annalisa Volpe, Elisabetta Primiceri, Caterina Gaudiuso, Antonio Ancona, Francesco Ferrara, and Maria Serena Chiriacò. 2021. "SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis" Micromachines 12, no. 8: 885. https://doi.org/10.3390/mi12080885