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Article

Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures

1
Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Ushinomiya-cho, Sakyo-ku, Kyoto 606-8501, Japan
2
Incubation Center Organs On Chip Project, Ushio INC, 1-6-5 Marunouchi, Chiyoda-ku, Tokyo 100-8150, Japan
3
Wuya College of Innovation, Shenyang Pharmaceutical University, Liaoning 110016, China
4
Department of Pharmaceutics, Shenyang Pharmaceutical University, Liaoning 110016, China
*
Author to whom correspondence should be addressed.
Academic Editor: Satoshi Fujita
Micromachines 2021, 12(5), 550; https://doi.org/10.3390/mi12050550
Received: 24 March 2021 / Revised: 8 May 2021 / Accepted: 10 May 2021 / Published: 12 May 2021
(This article belongs to the Special Issue Cell and Tissue Microdevices)
Microfluidic microphysiological systems (MPSs) or “organs-on-a-chip” are a promising alternative to animal models for drug screening and toxicology tests. However, most microfluidic devices employ polydimethylsiloxane (PDMS) as the structural material; and this has several drawbacks. Cyclo-olefin polymers (COPs) are more advantageous than PDMS and other thermoplastic materials because of their low drug absorption and autofluorescence. However, most COP-based microfluidic devices are fabricated by solvent bonding of the constituent parts. Notably, the remnant solvent can affect the cultured cells. This study employed a photobonding process with vacuum ultraviolet (VUV) light to fabricate microfluidic devices without using any solvent and compared their performance with that of solvent-bonded systems (using cyclohexane, dichloromethane, or toluene as the solvent) to investigate the effects of residual solvent on cell cultures. Quantitative immunofluorescence assays indicated that the coating efficiencies of extracellular matrix proteins (e.g., Matrigel and collagen I) were lower in solvent-bonded COP devices than those in VUV-bonded devices. Furthermore, the cytotoxicity of the systems was evaluated using SH-SY5Y neuroblastoma cells, and increased apoptosis was observed in the solvent-processed devices. These results provide insights into the effects of solvents used during the fabrication of microfluidic devices and can help prevent undesirable reactions and establish good manufacturing practices. View Full-Text
Keywords: microfluidic device; microphysiological systems; organs-on-a-chip; solvent bonding; cytotoxicity; photobonding; vacuum ultraviolet microfluidic device; microphysiological systems; organs-on-a-chip; solvent bonding; cytotoxicity; photobonding; vacuum ultraviolet
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MDPI and ACS Style

Wen, X.; Takahashi, S.; Hatakeyama, K.; Kamei, K.-i. Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures. Micromachines 2021, 12, 550. https://doi.org/10.3390/mi12050550

AMA Style

Wen X, Takahashi S, Hatakeyama K, Kamei K-i. Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures. Micromachines. 2021; 12(5):550. https://doi.org/10.3390/mi12050550

Chicago/Turabian Style

Wen, Xiaopeng, Seiichiro Takahashi, Kenji Hatakeyama, and Ken-ichiro Kamei. 2021. "Evaluation of the Effects of Solvents Used in the Fabrication of Microfluidic Devices on Cell Cultures" Micromachines 12, no. 5: 550. https://doi.org/10.3390/mi12050550

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