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Microfluidic Cell Transport with Piezoelectric Micro Diaphragm Pumps

1
Fraunhofer EMFT Research Institution for Microsystems and Solid State Technologies, Hansastrasse 27d, 80686 Munich, Germany
2
MAB-Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany
3
TranslaTUM—Central Institute for Translational Cancer Research, Technical University of Munich, Einsteinstrasse 25, 81675 Munich, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Amy Shen
Micromachines 2021, 12(12), 1459; https://doi.org/10.3390/mi12121459
Received: 22 October 2021 / Revised: 18 November 2021 / Accepted: 21 November 2021 / Published: 27 November 2021
(This article belongs to the Section B:Biology and Biomedicine)
The automated transport of cells can enable far-reaching cell culture research. However, to date, such automated transport has been achieved with large pump systems that often come with long fluidic connections and a large power consumption. Improvement is possible with space- and energy-efficient piezoelectric micro diaphragm pumps, though a precondition for a successful use is to enable transport with little to no mechanical stress on the cell suspension. This study evaluates the impact of the microfluidic transport of cells with the piezoelectric micro diaphragm pump developed by our group. It includes the investigation of different actuation signals. Therewith, we aim to achieve optimal fluidic performance while maximizing the cell viability. The investigation of fluidic properties proves a similar performance with a hybrid actuation signal that is a rectangular waveform with sinusoidal flanks, compared to the fluidically optimal rectangular actuation. The comparison of the cell transport with three actuation signals, sinusoidal, rectangular, and hybrid actuation shows that the hybrid actuation causes less damage than the rectangular actuation. With a 5% reduction of the cell viability it causes similar strain to the transport with sinusoidal actuation. Piezoelectric micro diaphragm pumps with the fluidically efficient hybrid signal actuation are therefore an interesting option for integrable microfluidic workflows. View Full-Text
Keywords: micro diaphragm pump; microfluidic; micro dosing; cell transport; automated cell culture; passive spring valves micro diaphragm pump; microfluidic; micro dosing; cell transport; automated cell culture; passive spring valves
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MDPI and ACS Style

Bußmann, A.; Thalhofer, T.; Hoffmann, S.; Daum, L.; Surendran, N.; Hayden, O.; Hubbuch, J.; Richter, M. Microfluidic Cell Transport with Piezoelectric Micro Diaphragm Pumps. Micromachines 2021, 12, 1459. https://doi.org/10.3390/mi12121459

AMA Style

Bußmann A, Thalhofer T, Hoffmann S, Daum L, Surendran N, Hayden O, Hubbuch J, Richter M. Microfluidic Cell Transport with Piezoelectric Micro Diaphragm Pumps. Micromachines. 2021; 12(12):1459. https://doi.org/10.3390/mi12121459

Chicago/Turabian Style

Bußmann, Agnes, Thomas Thalhofer, Sophie Hoffmann, Leopold Daum, Nivedha Surendran, Oliver Hayden, Jürgen Hubbuch, and Martin Richter. 2021. "Microfluidic Cell Transport with Piezoelectric Micro Diaphragm Pumps" Micromachines 12, no. 12: 1459. https://doi.org/10.3390/mi12121459

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