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Article

Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate

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Medical Research Institute, Kanazawa Medical University, Uchinada 920-0293, Japan
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Division of Translational and Clinical Oncology, Cancer Research Institute, Kanazawa University, Kanazawa 920-0934, Japan
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Ginrei Lab Inc., Uchinada 920-0293, Japan
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Department of Urology, Jikei University School of Medicine, Tokyo 105-0003, Japan
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Division of Cellular Signaling, National Cancer Center Research Institute, Tokyo 104-0045, Japan
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Shinko Chemical Co., Ltd., Kanazawa 920-0346, Japan
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Department of Head and Neck Surgery, Kanazawa Medical University, Uchinada 920-0293, Japan
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Department of Otolaryngology-Head and Neck Surgery, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan
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Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya 467-8601, Japan
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it4ip S.A., 1348 Louvain-la-Neuve, Belgium
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Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo 160-0023, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Ivan Cimrák
Micromachines 2021, 12(11), 1431; https://doi.org/10.3390/mi12111431
Received: 17 September 2021 / Revised: 14 November 2021 / Accepted: 16 November 2021 / Published: 21 November 2021
(This article belongs to the Special Issue Microfluidic Flow Cells: Modelling and Experiments)
Microfluidics is applied in biotechnology research via the creation of microfluidic channels and reaction vessels. Filters are considered to be able to simulate microfluidics. A typical example is the cell culture insert, which comprises two vessels connected by a filter. Cell culture inserts have been used for years to study cell-to-cell communication. These systems generally have a bucket-in-bucket structure and are hereafter referred to as a vertical-type co-culture plate (VTCP). However, VTCPs have several disadvantages, such as the inability to simultaneously observe samples in both containers and the inability of cell-to-cell communication through the filters at high cell densities. In this study, we developed a novel horizontal-type co-culture plate (HTCP) to overcome these disadvantages and confirm its performance. In addition, we clarified the migration characteristics of substances secreted from cells in horizontal co-culture vessels. It is generally assumed that less material is exchanged between the horizontal vessels. However, the extracellular vesicle (EV) transfer was found to be twice as high when using HTCP. Other merits include control of the degree of co-culture via the placement of cells. We believe that this novel HTCP container will facilitate research on cell-to-cell communication in various fields. View Full-Text
Keywords: co-culture plate; multiple connection; horizontal co-culture; extracellular vesicles; exosome; track-etched-membrane; metabolite co-culture plate; multiple connection; horizontal co-culture; extracellular vesicles; exosome; track-etched-membrane; metabolite
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MDPI and ACS Style

Shimasaki, T.; Yamamoto, S.; Omura, R.; Ito, K.; Nishide, Y.; Yamada, H.; Ohtomo, K.; Ishisaka, T.; Okano, K.; Ogawa, T.; Tsuji, H.; Matsuo, Y.; Minamoto, T.; Tomosugi, N.; Ferain, E.; Ochiya, T. Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate. Micromachines 2021, 12, 1431. https://doi.org/10.3390/mi12111431

AMA Style

Shimasaki T, Yamamoto S, Omura R, Ito K, Nishide Y, Yamada H, Ohtomo K, Ishisaka T, Okano K, Ogawa T, Tsuji H, Matsuo Y, Minamoto T, Tomosugi N, Ferain E, Ochiya T. Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate. Micromachines. 2021; 12(11):1431. https://doi.org/10.3390/mi12111431

Chicago/Turabian Style

Shimasaki, Takeo, Satoko Yamamoto, Risa Omura, Kagenori Ito, Yumiko Nishide, Hideki Yamada, Kazumi Ohtomo, Tomo Ishisaka, Keiichiro Okano, Takenori Ogawa, Hiroyuki Tsuji, Yoichi Matsuo, Toshinari Minamoto, Naohisa Tomosugi, Etienne Ferain, and Takahiro Ochiya. 2021. "Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate" Micromachines 12, no. 11: 1431. https://doi.org/10.3390/mi12111431

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