Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis

Abdul Sisak, Muhammad Asri; Louis, Fiona; Hyeok Lee, Sun; Chang, Young-Tae; Matsusaki, Michiya

doi:10.3390/mi11080727

Open AccessArticle

Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis

by

Muhammad Asri Abdul Sisak

¹,

Fiona Louis

²

,

Sun Hyeok Lee

^3,4,

Young-Tae Chang

^3,5 and

Michiya Matsusaki

^1,*

¹

Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

²

Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan

³

Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Korea

⁴

School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea

⁵

Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, Korea

^*

Author to whom correspondence should be addressed.

Micromachines 2020, 11(8), 727; https://doi.org/10.3390/mi11080727

Submission received: 13 July 2020 / Revised: 24 July 2020 / Accepted: 25 July 2020 / Published: 27 July 2020

(This article belongs to the Special Issue Cell Microarrays)

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Abstract

Conventional microarray analysis usually deals with the monolayer or two-dimensional (2D) assays for the high-throughput screening applications. Even though these cell-based assays are effective for preliminary screening at least to have information on cytotoxicity, they do not adequately re-create the in vivo complexity of three-dimensional (3D) tissues. In this study, 3D-blood capillary models were constructed by using physiological collagen microfibers (CMF), which provide the extracellular matrix in the complex tissue. Micro-droplets of fibrin gels containing CMF, endothelial cells, and fibroblasts were cultured for five days in 48-wells plate to provide a medium-throughput system for screening applications. Blood capillaries networks were formed by optimizing the concentration of CMF used and the number of cells. Finally, this screening method was a powerful assay for the application on the selection of not only a specific chemical probe for blood capillary live-imaging, but also a drug, aptamer, and peptide with potential blood vessel targeting property.

Keywords: blood capillary models; fibrin gels; collagen microfibers

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MDPI and ACS Style

Abdul Sisak, M.A.; Louis, F.; Hyeok Lee, S.; Chang, Y.-T.; Matsusaki, M. Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis. Micromachines 2020, 11, 727. https://doi.org/10.3390/mi11080727

AMA Style

Abdul Sisak MA, Louis F, Hyeok Lee S, Chang Y-T, Matsusaki M. Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis. Micromachines. 2020; 11(8):727. https://doi.org/10.3390/mi11080727

Chicago/Turabian Style

Abdul Sisak, Muhammad Asri, Fiona Louis, Sun Hyeok Lee, Young-Tae Chang, and Michiya Matsusaki. 2020. "Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis" Micromachines 11, no. 8: 727. https://doi.org/10.3390/mi11080727

APA Style

Abdul Sisak, M. A., Louis, F., Hyeok Lee, S., Chang, Y.-T., & Matsusaki, M. (2020). Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis. Micromachines, 11(8), 727. https://doi.org/10.3390/mi11080727

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis

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