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Open AccessArticle

Patterning of Particles and Live Cells at Single Cell Resolution

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The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel
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The Faculty of Engineering, Bar Ilan University, Ramat Gan 5290002, Israel
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Bar Ilan Institute of Nanotechnologies and Advanced Materials, Bar Ilan University, Ramat Gan 5290002, Israel
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Author to whom correspondence should be addressed.
Micromachines 2020, 11(5), 505; https://doi.org/10.3390/mi11050505
Received: 1 March 2020 / Revised: 12 May 2020 / Accepted: 13 May 2020 / Published: 15 May 2020
The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most of them deal with the patterning of cell populations, and are either not suitable or difficult to implement for the patterning of single cells. Here, we describe a bottom-up strategy for the micropatterning of cells and cell-sized particles. We have configured a micromanipulator system, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from different types, and positioning them with high accuracy in a predefined position, with a resolution smaller than 10 µm. Complementary DNA sequences were used to stabilize and hold the patterns together. The system is accurate, flexible, and easy-to-use, and can be automated for larger-scale tasks. Importantly, it maintains the viability of live cells. We provide quantitative measurements of the process and offer a file format for such assemblies. View Full-Text
Keywords: micropatterning; micromanipulator; single cell resolution; complementary DNA sequences micropatterning; micromanipulator; single cell resolution; complementary DNA sequences
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Hacohen, A.; Jessel, H.R.; Richter-Levin, A.; Shefi, O. Patterning of Particles and Live Cells at Single Cell Resolution. Micromachines 2020, 11, 505.

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