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Article

A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids

1
Cell Dynamics isrl, via Piero Gobetti 101, 40129 Bologna, Italy
2
Department of Pharmacy and Biotechnology (FaBiT), Laboratory of Human and General Physiology, Università of Bologna, via San Donato 19/2, 40127 Bologna, Italy
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Laboratory of Preclinical Studies for Regenerative Medicine of the Musculoskeletal System (RAMSES) Laboratory, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Ortopedico Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy
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Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Via Massarenti 9, 40138 Bologna, Italy
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National Research Council (CNR)-Institute of Molecular Genetics “Luigi Luca Cavalli-Sforza”, Unit of Bologna, 40136 Bologna, Italy
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IRCCS Istituto Ortopedico Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Micromachines 2020, 11(5), 465; https://doi.org/10.3390/mi11050465
Received: 23 March 2020 / Revised: 23 April 2020 / Accepted: 28 April 2020 / Published: 28 April 2020
(This article belongs to the Section E:Engineering and Technology)
Gathering precise information on mass density, size and weight of cells or cell aggregates, is crucial for applications in many biomedical fields with a specific focus on cancer research. Although few technical solutions have been presented for single-cell analysis, literature does not cover this aspect for 3D models such as spheroids. Since the research interest on such samples is notably rising, here we describe a flow-apparatus, and the associated physical method and operative protocol for the accurate measurements of mass density, size and weight. The technique is based on the detection of the terminal velocity of a free-falling sample into a specifically conceived analysis flow-channel. Moreover, in order to demonstrate the accuracy and precision of the presented flow-device, analyses were initially carried out on standardized polystyrene beads. Finally, to display the application of the proposed system for biological samples, mass density, size and weight of live SW620 tumor spheroids were analyzed. The combined measurements of such parameters can represent a step toward a deeper understanding of 3D culture models. View Full-Text
Keywords: mass density; spheroids; 3D cell culture models; multiparametric characterization; flow-reactors; analytical device mass density; spheroids; 3D cell culture models; multiparametric characterization; flow-reactors; analytical device
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MDPI and ACS Style

Cristaldi, D.A.; Sargenti, A.; Bonetti, S.; Musmeci, F.; Delprete, C.; Bacchi, F.; Pasqua, S.; Cavallo, C.; Bonsi, L.; Alviano, F.; Gazzola, D.; Santi, S. A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids. Micromachines 2020, 11, 465. https://doi.org/10.3390/mi11050465

AMA Style

Cristaldi DA, Sargenti A, Bonetti S, Musmeci F, Delprete C, Bacchi F, Pasqua S, Cavallo C, Bonsi L, Alviano F, Gazzola D, Santi S. A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids. Micromachines. 2020; 11(5):465. https://doi.org/10.3390/mi11050465

Chicago/Turabian Style

Cristaldi, Domenico A., Azzurra Sargenti, Simone Bonetti, Francesco Musmeci, Cecilia Delprete, Francesco Bacchi, Simone Pasqua, Carola Cavallo, Laura Bonsi, Francesco Alviano, Daniele Gazzola, and Spartaco Santi. 2020. "A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids" Micromachines 11, no. 5: 465. https://doi.org/10.3390/mi11050465

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