2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics
2. The Need for a Rapid Detection Method and Portable Detection Devices
3. A Potential Candidate for Rapid Detection of SARS-CoV-2: Loop-Mediated Isothermal Amplification (LAMP) Assays in PoC Devices
4. Other Important Factors in Fighting COVID-19
Conflicts of Interest
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|Coronaviruses (CoVs):||A large and diverse family of enveloped, positive-stranded RNA viruses, with a ~26–32 kilobase genome . The Coronaviridae cover a broad host range, infecting many mammalian and avian species, and induce upper respiratory, gastrointestinal, hepatic, and central nervous system diseases . In the last few decades, coronaviruses have been shown to be capable of also infecting humans. The outbreak of severe acute respiratory syndrome (SARS) in 2003, and, more recently, Middle-East respiratory syndrome (MERS) have proved the lethality of CoVs when they cross the species barrier and infect humans .|
|2019 novel coronavirus (SARS-CoV-2 ):||A new zoonotic human coronavirus, which was reported and announced by the Chinese Center for Disease Control and Prevention (CCDC) on 9 January 2020 . In spite of the fact that the initial infected cases have been associated with the Huanan South China Seafood Market, the source of COVID-19 is still unknown (Figure 1). On 30 January 2020, the WHO declared a global public health emergency regarding the outbreak of COVID-19. On the 11 March 2020, the WHO declared the outbreak of COVID-19 a pandemic.|
(Multiple heating and cooling cycle; hence, bulky and cumbersome).
|Isothermal and continuous amplification|
(Smaller, simpler, hence portable).
|Always requires sample concentration and preparation|
|For virus detection, for example, influenza  or human norovirus, LAMP assay offers one-step detection . Sample preparation steps are simplified.|
(Complicated and requires a skilled technician).
|Inhibitors hinder the reaction.||Tolerate inhibitors and more stable.|
|Diagnostic sensitivity (95%) is currently reported lower than LAMP [33,41,42].||Diagnostic sensitivity > 95%.|
|Established technique.||Applications using LAMP assays are being explored.|
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Nguyen, T.; Duong Bang, D.; Wolff, A. 2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics. Micromachines 2020, 11, 306. https://doi.org/10.3390/mi11030306
Nguyen T, Duong Bang D, Wolff A. 2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics. Micromachines. 2020; 11(3):306. https://doi.org/10.3390/mi11030306Chicago/Turabian Style
Nguyen, Trieu, Dang Duong Bang, and Anders Wolff. 2020. "2019 Novel Coronavirus Disease (COVID-19): Paving the Road for Rapid Detection and Point-of-Care Diagnostics" Micromachines 11, no. 3: 306. https://doi.org/10.3390/mi11030306